HLA DQB1 * 03 Allele | CIN 3 (2n = 224) | CIN 1 (2n = 132) | Controls (2n = 840) | Odds Ratio (95% CI) | χ2 Trend |
---|
0301 | 49 (21) | 25 (19) | 79 (9) | 2.53 (1.79–3.57) | 28.6 (p < 0.001) |
0302 | 45 (20) | 16 (12) | 85 (10) | 1.84 (1.29–2.62) | 15.5 (p < 0.001) |
0303 | 5 (2) | 7 (5) | 21 (2.5) | 1.36 (0.67–2.76) | 0.05 (p = 0.82) |
0304 | 0 | 0 | 1 (0.1) | 0 | Â |
Non-DQB1 * 03 | 125 | 84 | 654 | 1* | Â |
Total | 224 | 132 | 840 | Â | Â |
- The ARFLP-PCR technique used on DNA from cervical smears, following Mlu I digestion, identifies women who are negative, heterozygous, or homozygous for the DQB1 * 03 locus (Fig. 2). Of women with CIN, 38% were negative, 37% were heterozygous, and 23% were homozygous for the DQB1 * 03 locus (χ2 trend = 39.01, p < 0.001). Compared with controls, the odds ratio was greater for homozygosity (4.0, 95% CI 2.43–6.6) than for heterozygosity (2.63, 95% CI 1.75–3.94). Further typing of the DQB1 * 03 locus in positive samples showed that the 0301 allele was present in 40% of CIN as opposed to 9% of controls (odds ratio 2.53, 95% CI 1.79–3.57; χ2 trend = 28.6, p <0.001). DQB1 * 0302 was present in 32% and 10% of CIN and controls, respectively (odds ratio 1.84, 95% CI 1.29–2.62).