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Fig. 6 | Molecular Medicine

Fig. 6

From: Nuclear Localization Signal of HIV-1 as a Novel Target for Therapeutic Intervention

Fig. 6

PCR in situ hybridization analysis of HIV-1-infected monocytes

Monocytes were inoculated with 10 ng p24/106 cells of HIV-1ADA, washed after 2 hr absorption and incubated for additional 48 hr. One hundred nanomolar concentration of H-0294 was present in experimental samples at all steps of the procedure. At the end of a 48-hr incubation, culture medium was removed, cells were washed three times with PBS and fixed with 10% buffered formalin for 24 hr. Monocytes were scraped from the plate with rubber policemen and analyzed by in situ PCR as described in Materials and Methods. (A) Monocytes treated with H-0294; (B) untreated (control) infected monocytes; (C) negative control (without Taq polymerase). Large arrows point to the cytoplasm, and small arrows to nuclei.

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