- Original Articles
- Open Access
Identification of Galectin-3 As a High-Affinity Binding Protein for Advanced Glycation End Products (AGE): A New Member of the AGE-Receptor Complex
© Molecular Medicine 1995
- Published: 1 September 1995
Advanced glycation end products (AGE), the reactive derivatives of nonenzymatic glucose-protein condensation reactions, are implicated in the multiorgan complications of diabetes and aging. An AGE-specific cellular receptor complex (AGE-R) mediating AGE removal as well as multiple biological responses has been identified. By screening an expression library using antibody against a previously identified component of the AGE-R complex p90, a known partial cDNA clone was isolated with homology to galectin-3, a protein of diverse identity, and member of the galectin family.
Materials and Methods
To explore this unexpected finding, the nature of the interactions between galectin-3 and AGE was studied using intact macrophage-like RAW 264.7 cells, membrane-associated and recombinant galectin-1 through -4, and model AGE-ligands (AGE-BSA, FFI-BSA).
Among the members of this family (galectin-1 through 4), recombinant rat galectin-3 was found to exhibit high-affinity 125I-AGE-BSA binding with saturable kinetics (kD 3.5 × 107 M−1) that was fully blocked by excess unlabeled naturally formed AGE-BSA or synthetic FFI-BSA, but only weakly inhibited by several known galectin-3 ligands, such as lactose. In addition to the p90, immunoprecipitation with anti-galectin-3, followed by 125I-AGE-BSA ligand blot analysis of RAW 264.7 cell extracts, revealed galectin-3 (28 and 32 kD), as well as galectin-3-associated proteins (40 and 50 kD) with AGE-binding activity. Interaction of galectin-3 with AGE-BSA or FFI-BSA resulted in formation of SDS-, and β-mercaptoethanol-insoluble, but hydroxylamine-sensitive high-molecular weight complexes between AGE-ligand, galectin-3, and other membrane components.
The findings point toward a mechanism by which galectin-3 may serve in the assembly of AGE-R components and in the efficient cell surface attachment and endocytosis by macrophages of a heterogenous pool of AGE moieties with diverse affinities, thus contributing to the elimination of these pathogenic substances.
Glucose and other reducing sugars react spontaneously with a wide spectrum of proteins in vitro and in vivo to initiate a post-translational modification process, the late products of which comprise of a heterogeneous group of irreversible adducts called advanced glycation end products (AGE) (1, 2). In vivo formation of AGE-proteins proceeds slowly under normal ambient glucose concentrations, while the rate of AGE accumulation is enhanced in the presence of hyperglycemia, as in diabetes mellitus (3, 4). Numerous studies suggest that AGEs play an important role in the structural and functional alterations that occur in long-term diabetes and more slowly in normal aging (1–5).
Binding and internalization of AGE-modified proteins is facilitated through specific cell surface receptors identified first on cells of the monocyte/macrophage lineage (6, 7) and subsequently on endothelial (8), mesangial cells (9) and other cell types (4, 10). In addition to the uptake and degradation of senescent AGE-modified proteins by macrophages, AGE-receptor/ligand interactions initiate a range of biologically important cellular responses, including chemotaxis, activation, cytokine, and growth factor secretion (4, 5). Based on these properties, cellular AGE-receptors are thought to contribute to normal growth patterns and tissue turnover.
Initial studies to determine the molecular composition of AGE receptors revealed two AGE-binding polypeptides at approximately 30 and 90 kD, based on AGE-specific affinity precipitation of radiolabeled cell surface proteins from the murine macrophage cell line, RAW 264.7 (11, 12). Subsequently, two AGE-binding proteins, designated p60 and p90, were isolated from rat liver membranes and partially sequenced (13). Antisera raised against purified p60 and p90 recognized surface determinants and blocked AGE binding and AGE-dependent responses of human monocytes/macrophages (13), rat T cells (14), and murine mesangial cells (15). This broad range of activity across cellular systems and species suggested that this AGE-receptor system involves highly conserved proteins. Additional AGE-binding proteins, a 35-kD protein, named RAGE, and an 80-kD protein homologous to lactoferrin were also described (16, 17), further expanding this novel class of molecules. The molecular mechanisms by which single AGE-binding proteins associate with other membrane proteins, how they form complexes with them or how they specifically recognize various members of the highly heterogeneous family of AGE structures is unknown. AGE-receptor ligand interactions are only weakly inhibited by free carbohydrates or by proteins modified by early glycation products such as the Amadori adduct (6, 8, 9, 14).
Other mammalian proteins with lectin-like affinity for a spectrum of sugars and glycoconjugates have been described (18, 19). Among them, galectins consist of a well defined family of molecules sharing characteristic amino acid sequences and affinity for β-galactoside sugars (19, 20). Of these, galectin-3, first described as a cell surface marker for activated macrophages (Mac-2) (21), was subsequently identified as a lactose-specific lectin present on various cells and cellular compartments (22–25). The cDNA and the protein product have been characterized by several laboratories, reporting it as a 35-kD carbohydrate-binding protein (CBP35), a nuclear protein with a role in cell cycle regulation (22); a 32-kD molecule isolated from rat basophilic leukemia cells that binds rat IgE through carbohydrate moieties (26), and as a 29-kD lung lectin with lactose specificity (HL29) (25). The principal physiological role of this molecule in humans remains unknown.
In this report, we describe the identification of the polypeptide currently termed galectin-3 as a macrophage cell membrane protein that exhibits high-affinity binding for nonenzymatically glycated (AGE)-modified proteins and which facilitates covalent complex formation with these ligands.
Chemicals and Reagents
Recombinant galectin-3 (rat, and murine CBP-35) was prepared as previously described (26). C-terminal domain peptide (18 kD) of murine galectin-3 was a generous gift from Dr. John L. Wang (Michigan State University, East Lansing, MI, U.S.A.). Recombinant human galectin-1 and -2, and rat galectin-4 (domain I) were provided by Drs. H. Leffler and S. H. Barondes (University of California, San Francisco), bovine serum albumin (BSA) (Fraction V, low endotoxin), Nonidet P-40 (NP-40), and Triton X-100 were purchased from Boehringer-Mannheim Biochemicals (Indianapolis, IN, U.S.A.). Glucose, lactose, galactose, ovalbumin, hydroxylamine, hydrazine, 2-PAM and glycoconjugates were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). Sodium 125I (s.a. 10 mCi/100 µl) was obtained from New England Nuclear (Boston, MA, U.S.A.). Nitrocellulose membranes were from Schliecher & Schuell (Keene, NH, U.S.A.). The chemically synthesized AGE, 2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole hexanoicacid (FFI-HA) (27, 28) was generously provided by Dr. Peter Ulrich (The Picower Institute, NY, U.S.A.). Pyrraline and pentosidine were a gift of Dr. Vincent Monnier (Case Western Reserve, CL).
Preparation and Radiolabeling of Ligands
AGE-modified bovine serum albumin (AGE-BSA) and ovalbumin (AGE-Ova) were made by incubating each protein with 0.5 M glucose at 37°C for 6 weeks in phosphate-buffered saline (400 mM, pH 7.4), as previously described (13, 15). Unincorporated glucose was removed by dialysis against PBS. AGE-modification was measured by an AGE-specific ELISA (29). AGE-BSA contained approximately 300 AGE U/mg, AGE-OVA 1000 AGE U/mg, and BSA contained <3 U/mg.
The chemically defined model AGE, FFI-HA, was synthesized and linked to BSA as described previously (27). Ligand radioiodination was performed with carrier-free-125I using IODO-beads (Pierce, Rockford, IL, U.S.A.) according to the manufacturer’s instructions.
Cells of the murine macrophage-like cell line, RAW 264.7 were cultured in monolayers and were collected by gentle scraping and washing in phosphate buffered saline (PBS), centrifuged at 500 × g for 5 min, disrupted with a tight Dounce homogenizer, in a solution of PBS containing 1 mM EDTA and protease inhibitors, 2 mM phenyl-methyl-sulfonyl-fluoride (PMSF), 10 µg/ml aprotinin, 5 ng/ml pepstatin, 1 mM benzamidine. Nuclei and cell debris were removed by centrifugation at 1000 × g for 10 min. The cellular membranes were isolated from the supernatant by centrifugation at 10,000 × g for 20 min at 4°C. The resulting membrane-enriched fraction was solubilized in PBS containing 0.5% NP-40, and protease inhibitors as described above. This material was then used for ligand and Western blot studies. In addition, whole cell extracts were prepared by lysing cells in PBS containing 0.5% NP-40 and protease inhibitors. After 10 min incubation on ice, nuclei and cell debris were removed by centrifugation at 10,000 × g for 10 min. Protein concentrations were determined by BCA protein assay (Pierce).
Ligand and Western Blotting
In most studies described below, pure recombinant murine galectin-3 or cell membrane preparations were mixed with an equal volume of Laemli 2 × SDS-PAGE sample buffer containing 5% 2-mercaptoethanol, electrophoresed on 12% SDS-PAGE, and then electroblotted onto a nitrocellulose filter, as previously described (30). For ligand blot analysis, following blocking for 1 hr in a solution of PBS containing 1.5% BSA and 0.1% Triton X-100, 1 mM MgCl2 and 1 mM CaCl2, the nitrocellulose filters were probed 125I-AGE-BSA (300 nM) in blocking solution in the presence or absence of various competitors. The blots were washed three times with PBS containing 0.1% Triton X-100 and exposed to Kodak XAR-5 film at −80°C. Quantitation of bound radioactivity was performed on a Molecular Dynamics phosphorimager and the values were expressed as relative phosphorimage units. For Western blot analysis, following blocking with PBS containing 2% BSA, electroblotted proteins were probed with various primary antibodies as indicated and visualized by using alkaline phosphatase-conjugated secondary antibodies and the NBT/BCIP western blot detection method (31). In separate studies, recombinant human galectin-1, -2, and -3, and rat galectin-4 (domain I) were subjected to SDS-PAGE through a 4-20% gel (5 µg/lane) and electroblotted onto nitrocellulose membranes. Filters were either stained with Amido black or probed with 125I-AGE-BSA.
Avian anti-rat polyclonal antibodies, raised against purified rat liver AGE-binding proteins p60 and p90, were prepared as described (13). Rat monoclonal antibody (mAb) specific to murine galectin-3 was purified from culture supernatants of hybridoma M3/38 (ATCC TIB 166) using protein G-sepharose column (Boehringer-Mannheim Biochemicals). Isotypic rat IgG2a was purchased from Zymed Immunochemicals (South San Francisco, CA, U.S.A.). A polyclonal antibody to CBP-35 was generously provided by Dr. J. L. Wang, Michigan State University.
Radiolabeling, Immuno-Precipitation and Ligand-Precipitation
RAW 264.7 cells (1 × 107) were surface radiolabeled using lactoperoxidase-catalyzed iodination as described (30). After surface iodination, detergent-solubilized whole cell extracts were pre-cleared by incubation with BSA-Sepharose for 1 hr at 4°C. For immunoprecipitation, 2.5 µg of mAb M3/38 or the isotype control (rat IgG2a) were added and the incubation was extended for 14 hr at 4°C with gentle rocking. To isolate the Ab-Ag complexes, goat anti-rat antibodies, linked to agarose beads (Sigma) were added to the incubation. After an additional 2 hr, the Ab-Ag complexes were isolated by centrifugation at 2,000 × g and washed four times with PBS containing 0.5% NP-40. An equal volume of Laemli SDS-PAGE buffer containing 2.5% β-mercaptoethanol was added to the obtained pellets. For ligand affinity precipitation, AGE-BSA was coupled to activated Sepharose-4B as described (13). After preclearing the cell extracts with BSA-Sepharose, AGE-BSA-Sepharose was added and the incubation was extended for 1 hr at 4°C with gentle rocking. The complexes were separated by centrifugation at 2000 × g and washed extensively with PBS containing 0.5% NP-40. An equal volume of Laemli SDS-PAGE buffer containing 2.5% β-mercaptoethanol was added to the washed ligand affinity precipitate. Following boiling for 2 min, the proteins were electrophoresed through a 12% SDS-PAGE gel. Ligand- and immuno-precipitated proteins were visualized by autoradiography.
Recombinant murine galectin-3, M3/38-immunoprecipitated galectin-3 or NP-40 detergent-solubilized cell membrane preparations were mixed with AGE-BSA or FFI-BSA at 1 µg/ml in PBS containing 1 mM CaCl2 in the presence or absence of hydroxylamine (50 mM), hydrazine (100 mM) or 2-PAM (50 mM). The aggregates were visualized by autoradiography of dried gels and the amount was quantitated by phosphor-image analysis.
RAW 264.7 cells were plated on coverslips coated with BS A at 1 mg/ml and were grown in DMEM for 2 days. Prior to assay, the medium was removed and replaced with 1 ml of fresh medium in the presence or absence of 50 mg/ml AGE-BSA. After the incubation, the cells were fixed in 3.5% formalin in PBS for 10 min at room temperature, blocked in PBS containing 0.1% BSA and 0.02% sodium azide for 15 min. Anti-galectin-3 and isotype control mAb were added to the cells and the incubation was allowed to continue for 1 hr. After washing the cells four times in PBS, FITC-conjugated goat anti-rat IgG (Fab)2 was added to the cells and incubated for 1 hr at room temperature. The cells were then washed four times in PBS and photographed.
Antibody to the AGE-Binding Protein p90 Recognizes Galectin-3
Recombinant Galectin-3 Binds AGE-Modified Proteins
To determine the domain responsible for AGE binding, recombinant galectin-3 was digested with collagenase (32, 33) and the resulting 18-kD C terminus was used for ligand blot analysis. As shown in Fig. 4B, this portion of the molecule bound 125I-AGE (Lane 1) in a manner that was inhibited in the presence of 50-fold excess unlabeled AGE-BSA (Lane 2), indicating AGE-binding specificity. In addition, when compared to the intact protein, the C-terminal fragment of galectin-3 exhibited stronger AGE-binding activity (Fig. 4 C and D); phosphorimage analysis indicated that the C terminus while retaining full AGE binding activity, as the intact protein, this is considerably higher than the binding activity of intact galectin-3. The finding suggests that the C terminus may contain the principal AGE-binding site domain of galectin-3 and the removal of collagen-like domain may facilitate the access of AGE-BSA ligand to this binding domain.
Carbohydrates Are Not Effective Competitors of AGE-Binding to Galectin-3
Cell-Surface Galectin-3 Binds AGE-BSA
AGE-BSA Treatment Causes Redistribution of Galectin-3 on the Cell Surface
Interaction of AGE-Ligand with Cell Surface Proteins Results in Formation of High-Molecular Weight Aggregates
Initially, monocyte/macrophages and subsequently other cell systems were found to bind AGE-modified substrates with high-affinity saturable kinetics, implying the existence of specific receptor(s) (6–9). This binding is not antagonized by the presence of simple sugars, glycoconjugates or early glycation reaction products, such as Schiff bases or Amadori products in large molar excess, nor by proteins modified by formaldehyde, acetylation or maleylation, which define in part the so-called scavenger receptors (6–9, 13, 14). AGE-receptor interactions have been linked to a wide variety of normal potentially pathogenic responses, including migration, activation, cytokine and growth factor induction, and matrix protein secretion (4, 5, 8, 9, 14, 15). These findings have further encouraged the identification and isolation of AGE-binding proteins and the molecular definition of the AGE-receptor complex (11–13, 16, 17).
Derived from the covalent attachment of reducing sugars, the advanced glycation products that form spontaneously on proteins or lipid substrates in vivo represent a structurally heterogeneous class of reactive adducts that can form infra- and intermolecular crosslinks (1–5). One such chemically defined AGE structure is the furoyl-furanyl imidazole (FFI), the first AGE-crosslink identified in vitro (27) as well as in animal tissues (28), while others subsequently isolated include the pyrrole containing AFGP (34), the pentosidine (35), and pyrraline (36); however, the large majority of AGE structures remains undefined.
We have previously described two polypeptides, designated p60 and p90, as components of the macrophage AGE-receptor (13). During studies designed to provide cDNA clones corresponding to p90 using antibodies made to purified rat liver p90, we repeatedly isolated a partial cDNA clone corresponding to galectin-3.
Galectin-3, a cellular protein previously studied for its relatively low binding activity for a broad range of simple carbohydrates (Kd 4 × 105 M−1) (37) is found in the cell nucleus, the cytosol, on the cell surface, as well as extracellularly and has been implicated in events such as macrophage migration, adhesion, immune response modulation, growth and differentiation and is included to the Galectins, a family of lectins, sharing affinity for β-galactoside sugars (18–26). There has been no clear evidence however that galectin-3 acts as a surface receptor, since protein and cDNA sequence analysis of this molecule indicated neither transmembrane anchor sequences, nor signal peptide (38). Western blot analysis of recombinant murine galectin-3, using the same antisera developed against the rat liver 90-kD AGE-binding polypeptide (anti-p90) (13) that were used in expression screening revealed strong immunoreactivity between these antisera and galectin-3. This raised the possibility of either an immunologic cross-reactivity between p90 and galectin-3 or of immunoreactivity directed to galectin-3-like epitopes copurified with the p90 polypeptide.
The physical association of galectin-3 with AGE-receptor components versus an independent role of galectin-3 as an AGE-binding protein was investigated further. First, it was ascertained that galectin-3 possessed distinct binding affinity for AGE-modified albumin, a property which was not shared by two other members of the galectin family (galectin-1 and -2) and only partially by galectin-4. Then, evidence was obtained to show that purified recombinant galectin-3 is capable of independently and specifically binding AGE-modified proteins with saturable kinetics (Kd = 3.5 × 107 M−1). This binding affinity was lower than the affinity observed in intact membrane preparations (×10−9) (13), which may reflect either the need for a membrane localization or for cooperation of more than one of the AGE receptor components. Nevertheless, the affinity of galectin-3 for AGEs is distinctly higher than that for carbohydrates (Kd = 4 × 105 M−1) (37), even when FFI-HA is used for comparison with monovalent sugars. In addition, based on competitive studies with previously known ligands of galectin-3 such as lactose, galactose, and other glycoconjugates added in large excess, the recognition site for AGE-moieties appeared to be distinct from those for carbohydrate moieties. At least one AGE-binding domain colocalizes to the same 18-kD C-terminal fragment of galectin-3 that contains the carbohydrate recognition domain of the molecule. Although our data do not distinguish between a single or multiple binding sites for AGE-modified ligands on galectin-3, the specificity of this protein for AGE-moieties was further defined in experiments using chemically defined synthetic “early” or Amadori, and “late” glycation intermediates (1–4) in competition experiments. Consistent with reports on lack of recognition by other AGE-binding proteins (6, 8, 13, 14), Amadori failed here to displace AGE-ligands, thus eliminating the role of early glycation products in this interaction. In contrast, the model synthetic AGE ligands, FFI-HA and FFI-BSA (25, 26), previously shown to be readily recognized by macrophages (6, 7, 11, 12) competed very effectively. Surface binding of FFI-BSA has been associated with macrophage/monocyte activation, cytokine release (39) and autocrine AGE-receptor up-regulation (40), mimicking and even surpassing the identical activities of naturally formed AGE-BSA. Owing its efficiency most probably to the higher concentration of AGE-modifications per polypeptide compared to the naturally formed AGE-BSA, the validity of the synthetic FFI-BSA was confirmed while it also proved a valuable probe for the present studies.
AGE-affinity precipitation of surface-labeled cell extracts yielded polypeptides with identical mobility to galectin-3 at 35 and 90 kD, while immunoprecipitation with anti-galectin-3 revealed in addition, a 50-kD protein, which indicated the proximity, and possible association of galectin-3 with other AGE-binding polypeptides on the cell surface (e.g., p90 and p60). Alternatively, exposure of cells to AGEs may enhance the surface expression of galectin-3, an antigen, first recognized as a macrophage activation marker (21). Given the known property of AGEs to induce macrophage activation (39, 40), this would not be inconsistent with this hypothesis. The latter was supported by immunofluorescence studies showing that upon exposure of RAW cells to AGE-BSA, the presence of galectin-3 on the cell surface intensified, while it mobilized to form discrete complexes. Based on the previously reported propensity of galectin-3 to associate with itself (18, 32, 37), we sought to evaluate whether AGE ligands may also promote galectin-3 complex formation.
Our results indicate that interaction of AGE-and FFI-modified BSA with either membrane-derived or recombinant galectin-3 resulted in the formation of high-molecular weight aggregates in a time-dependent manner. These complexes proved resistant to SDS or β-mercaptoethanol treatment but were completely inhibitable in the presence of nucleophiles, such as hydroxylamine, hydrazine, and 2-PAM. The lability of the complexes to hydroxylamine at an alkaline pH suggested that the interactions within the complexes may involve the formation of covalent bond between galectin-3 and other molecules, and that the bond is likely a thioester. The presence of such thioester linkages has been previously reported for complement factor C3 (41, 42, 43) and ubiquitin-activating enzyme E1 (44). Internal thioester bonds mediating the fixation of complement factor C3 to biological targets seem necessary for complement function (42, 43). Similarly, the attachment of ubiquitin El and subsequent trafficking of target proteins to degradation pathways is also dependent on high energy thioester linkages (44). Although further characterization of the nature and the functional significance of the linkages formed between galectin-3 and AGE-modified proteins is necessary, it is possible to speculate that the formation of high energy thioester bonds may contribute to the efficient attachment, and uptake of proteins modified in vivo by a wide range of AGE moieties with variable affinities, to be subsequently escorted toward intracellular degradative compartments.
The presence of galectin-3 on the cell surface is interesting since there is no consensus transmembrane spanning domain present in its sequence (18, 38). Thus, it is conceivable that this polypeptide is expressed on the cell surface in association with other subunits of the AGE-receptor complex which may contain such domains. In this regard, our immuno-precipitation and affinity studies using anti-galectin-3 and ligand blot analysis, showed that in addition to the p90 and to anti-galectin-3-reactive polypeptides, two other species with approximate molecular weights of 40 and 50 kD were associated with galectin-3 and were capable of binding AGE-BSA.
The identity of these molecules is under investigation; however, they are likely to correspond to one or more of the already characterized macrophage surface AGE-binding proteins (13, 16). Thus far, the primary role of galectin-3 has remained unclear, although it has been suggested that galectin, among others, may play a role in macrophage adhesion to basement membranes and subsequent migration toward the site of inflammation via interaction with laminin (45), or that it may be associated with neoplastic progression of colon carcinoma (46).
Collectively, our data provides evidence of a novel class of in vivo forming ligands for galectin-3, advanced glycation end products. The physiologic significance of this interaction is under further investigation. However, the observation that galectin-3 exhibits high-affinity binding activity for AGE-modified proteins, constitutes a novel activity for this molecule and introduces it as a new member of the macrophage receptor system for AGE-modified senescent macromolecules.
We would like to thank Dr. Kirk Manogue and Dr. Bradley Berger for their help and guidance. We also thank Dr. Hakon Leffler and Dr. Samuel H. Barondes (University of California, San Francisco, CA) for the supply of the galectin 1–4 and their valuable cooperation. Our thanks to Dr. J. L. Wang (Michigan State University) for providing CBP-35, and C-peptide and to Dr. Vincent Monnier (Case Western Reserve, CL) for pyrraline and pentosidine which were also generously provided. These studies were supported in part by The National Institutes of Health Grants AGO-6943 and AGO-9453 to HV.
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