Fig. 5
Effect of carbohydrates on AGE-binding to galectin-3
Anti-Galectin-3 immunoprecipitated material from RAW 264.7 cells (2 × 105) was subjected to SDS-PAGE (12%) and ligand blot analysis using 125I-AGE-BSA (300 nM) in the presence or absence or the indicated unlabelled competitors: AGE-BSA, FFI-BSA, FFI-HA at 50-fold excess (15 µM), lactose, galactose, galactosamine, and glucosamine at 100 mM, and Amadori product (1-deoxy-1-propyl-amino-fructose) at 1 mM. The amount of radioactivity associated with galectin-3 was determined by phosphorimage analysis of three identical experiments and expressed as percentage (mean ± SD) of 125I-AGE-BSA bound in the absence of competitors.