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Fig. 9 | Molecular Medicine

Fig. 9

From: Identification of Galectin-3 As a High-Affinity Binding Protein for Advanced Glycation End Products (AGE): A New Member of the AGE-Receptor Complex

Fig. 9

Phosphorimage analysis of high-molecular weight aggregates forming during galectin-3 and AGE-ligand interaction

After radioiodination, galectin-3 immunoprecipitated from RAW 264.7 cell membranes were incubated for 24 hr at 37°C alone (a) or with unmodified-BSA (b). Similar preparations were incubated with AGE-BSA (c) or with FFI-BSA (e) alone or in the presence of hydroxylamine (100 mM) (d and f). High-molecular weight complex formation was quantitated by phosphorimage (PI) analysis and expressed as PI U/mg protein. Data are representative of three independent experiments.

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