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Fig. 4 | Molecular Medicine

Fig. 4

From: Interleukin 1 Induces HIV-1 Expression in Chronically Infected U1 Cells: Blockade by Interleukin 1 Receptor Antagonist and Tumor Necrosis Factor Binding Protein Type 1

Fig. 4

IL-1 increases NF-κB DNA binding activity in U1 cells and IL-1Ra blocks IL-1-induced NF-κB binding activity

Ul cells were stimulated with IL-1β (10 ng/ml) and cultured for 3 hr in the presence of IL-1Ra (1 µg/ml). Gel mobility shift assays for NF-κB DNA binding activity were performed using 20 µg of nuclear extracts, a NF-κB probe and a NF-κB consensus sequence as a competitor (20 ng). The arrowhead indicates the region of NF-κB binding. (Lane 1) Unbound probe; (Lane 2) unstimulated U1 cells; (Lane 3) unstimulated U1 cells with NF-κB competitor sequence; (Lane 4) U1 cells with IL-1; (Lane 5) U1 cells with IL-1 and competitor; (Lane 6) U1 cells with IL-1Ra; (Lane 7) U1 cells with IL-1Ra and competitor; (Lane 8) U1 cells with IL-1 and IL-1Ra; (Lane 9) U1 cells with IL-1, IL-1Ra and competitor.

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