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Fig. 6 | Molecular Medicine

Fig. 6

From: Interleukin 1 Induces HIV-1 Expression in Chronically Infected U1 Cells: Blockade by Interleukin 1 Receptor Antagonist and Tumor Necrosis Factor Binding Protein Type 1

Fig. 6

Anti-IL-1RII monoclonal antibody blocks the binding of radiolabeled IL-1 to U1 cells, but does not inhibit IL-1-induced p24 antigen production

Ul cells (107) were preincubated for 2 hr at 4°C with different concentrations of anti-IL-1RII antibody prior to the addition of ∼100 pM 125IL-1α. After another 2 hr at 4°C, specific binding was determined. Nonspecific binding (which was measured in the presence of 10 µM of IL-1Ra) was 37% of total binding. The inset shows U1 cells preincubated with RPMI or 10 µg/ml anti-IL-1RII prior to stimulation with 50 ng/ml IL-1α. p24 antigen is depicted as the mean ± SD of four samples.

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Molecular Medicine

ISSN: 1528-3658