Fig. 3
From: Expression of TNF and TNF Receptors (p55 and p75) in the Rat Brain after Focal Cerebral Ischemia
Double-immunofluorescence for TNF and GFAP in the ischemic cortex
Double immunofluorescence for TNF (A, C; red filter) and GFAP (B, D; green filter) shows the colocalization of TNF and GFAP at the brain surface (A, B) and surrounding brain capillaries (C, D); in each case, the zone comprises astrocytic end-feet. No such colocalization was observed on the larger, noncapillary brain vessels (arrows on A, B), which also do not have an enveloping layer of astrocytic processes. Note colocalization of TNF and GFAP IR on small capillaries (arrows on C, D).