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Table 1 β-galactosidase activity g) of COS-7 cells transfected with pCMVβ (20, 40, 80, and 160 µg/ml) and histone H2A (0.03125, 0.0625, 0.125, 0.25, 0.5, 1, 2, and 4 mg/ml)

From: Histone H2A Significantly Enhances In Vitro DNA Transfection

Histone H2A mg/ml (µM)

DNA 160 µg/ml (0.0337/µM)

DNA 80 µg/ml (0.0168 µM)

DNA 40 µg/ml (0.0084, µM)

DNA 20, µg/ml (0.0042, µM)

0

4.0 (286.5)

7.80 ± −0.60

4.94 ± 0.35

2.69 ± 2.01

0.55 ± 0.028

 

2.0 (143.3)

9.54 ± 1.17

7.92 ± 3.38

0.19 ± 0.14

0.074 ± 0.073

 

1.0 (71.6)

0.93 ± 0.17

158.23 ± 18.65

4.70 ± 2.80

−0.23 ± 0.022

 

0.5 (35.8)

2.03 ± 0.45

0.93 ± 0.10

195.18 ± 8.77

37.50 ± 1.87

 

0.25 (17.9)

0.37 ± 0.10

0.65 ± 0.20

−0.56 ± 0.17

10.80 ± 3.13

 

0.125 (8.95)

0.73 ± 0.23

0.88 ± 0.50

−0.62 ± 0.06

1.22 ± 1.37

 

0.0625 (4.48)

0.65 ± 0.22

0.98 ± 0.38

−0.93 ± 0.23

−0.59 ± 0.27

 

0.03125 (2.24)

1.04 ± 0.47

0.81 ± 0.18

−1.01 ± 0.23

−1.18 ± 0.12

 

0

−0.11 ± 0

0.42 ± 0.50

−0.09 ± 0.06

0.65 ± 0.17

0.003 ± 2

  1. The combinations giving the most efficient transfection are shown in bold. The values shown are the means of triplicates ± 1 S.E.