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Fig. 2 | Molecular Medicine

Fig. 2

From: Functional Analysis of a Human Tumor Necrosis Factor α (TNF-α) Promoter Polymorphism Related to Joint Damage in Rheumatoid Arthritis

Fig. 2

Functional analysis of the pXP1-TNF-238G, pXP1-TNF-238A, or pXP1-TNF-238A-376A plasmids in U937 (A) and Mono Mac 6 cells (B). Twenty-four hours after transfection, U937 and Mono Mac 6 were mock stimulated (NS) or induced for 4 hr with LPS/PMA and LPS, respectively. Shown here are the means of three separate transfections with two different batches of plasmid DNA. The luciferase constructs were always tested simultaneously. Luciferase activity of the constructs is expressed as the relative activity of the plasmid with the highest activity (100%) reached at 4 hr after stimulation in each experiment. Relative luciferase activities (± SD) were averaged and plotted in histograms as depicted.

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