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Fig. 3 | Molecular Medicine

Fig. 3

From: Functional Analysis of a Human Tumor Necrosis Factor α (TNF-α) Promoter Polymorphism Related to Joint Damage in Rheumatoid Arthritis

Fig. 3

Functional analysis of the TNF-238G/CAT, TNF-238A/CAT, or TNF-238A/-376A/CAT plasmids in Jurkat T cells (A) and Raji B cells (B). Twenty-four hours after transfection, Jurkat and Raji cells were mock-stimulated (NS) or induced for 24 hr with αCD3/PMA and PMA, respectively. Shown are the means of six (Jurkat) and three (Raji) separate transfections with three different hatches of plasmid DNA. The CAT constructs were always tested simultaneously. CAT activity of the plasmids is expressed as the relative activity of the plasmid with the highest activity (100%) reached at 24 hr after stimulation in each experiment. Relative CAT activities (± SD) were averaged and plotted in histograms as depicted.

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