Fig. 3From: The Cytoplasmic Domain of Alzheimer’s Amyloid Precursor Protein Is Phosphorylated at Thr654, Ser655, and Thr668 in Adult Rat Brain and Cultured Cells Specificity of phosphorylation state-specific antibodies towards enzymatically phosphorylated APP 645–694 APP645–694 (6 µg) was incubated with nonradioactive ATP and CaM kinase II (CaMk), PKC, or without enzymes (−). Samples were subjected to SDS-PAGE, and transferred to nitrocellulose membranes. Membranes were probed with pAbT654 (lanes 1 to 3), pAbS655 (lanes 4 to 6), or G-369 (lanes 7 to 9) antibodies, followed by [125I]-protein A, and autoradiography was performed. The arrow indicates the position of dephospho-APP645–694.Back to article page