Fig. 2From: Normal Human Epidermal Keratinocytes Express In Vitro Specific Molecular Forms of (Pro)Filaggrin Recognized by Rheumatoid Arthritis-Associated Antifilaggrin Autoantibodies Double indirect immunoflorescence analysis of cultured human keratinocytes with AKH1, a MAb to (pro)filaggrin, and an RA serum The labeling of AKH1 is revealed with tetramethylrhodamine isothiocyanate-conjugated anti-mouse IgG (A, C) and that of the RA serum with fluorescein isothiocyanate-conjugated anti-human IgG (B, D). The MAb and the RA serum label the same granules (arrows). Dotted lines denote the basal limit of the cultured epithelial sheets. Bar = 200 µm (A, B); 50 µm(C, D).Back to article page