Fig. 4
From: Differentiation of Myeloid Cell Lines Correlates with a Selective Expression of RIZ Protein
Immunochemical analysis of RIZ protein. (A) HL60 cells were incubated in the presence (frames c and d) or absence (frames a–f) of 1 µM of ATRA. Frames a–d: staining with antibody to RIZ protein RZ1723. Frames e and f staining with antibody to PCNA. Frames b, d, and f: permeabilization with Triton X-100 followed by staining with the specific antibody. (B) Mononucleated cells from acute promyelocytic leukemia patient were incubated in the presence (frames b, e, and f) or absence (frames a, c, and d) of 1 µM of ATRA. Frames a and b: May-Grünwald staining. Frames c, d, e, and f immunoperoxidase staining with antibody to RIZ protein RZ1723.