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Fig. 1 | Molecular Medicine

Fig. 1

From: Identification of mRNAs Differentially Expressed in Quiescence or in Late G1 Phase of the Cell Cycle in Human Breast Cancer Cells by Using the Differential Display Method

Fig. 1

Synchronization of 21PT cells in G0 and in late G1 with two different approaches

(A) Exponentially growing asynchronous 21PT cells (control) were incubated in serum-deprived medium for 85 hr (SS). After serum starvation, cells were released into α-MEM medium containing 10% FBS with 400 µM mimosine for 24 hr (SS+mimo). Following mimosine block cells were shifted into complete medium without mimosine for 4 hr (SS+mimo+C). (B) Following serum starvation for 85 hr cells were released into complete medium at 0 hr. Samples are shown at 12, 14, and 24 hr after serum induction. (C) Cell cycle progression following serum induction of G0 cells. Samples were taken at indicated time points and monitored by FACS analysis as described in Materials and Methods.

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Molecular Medicine

ISSN: 1528-3658