Fig. 2

Identification and expression of cDNA fragments #1, #34, and #40, which are differentially expressed in G0 or in late G1

Mimosine was used for late G1 synchronization (Set 1). Differentially expressed pattern of #1 (A), #34 (E), and #40 (H) on the DD gel. Northern blot confirmation of #1 (B), #34 (F) and #40 (I). After fragments #1, #34, and #40 were cut from DD gel and PCR amplification with the corresponding primers used in DD, Northern blot analysis was performed as described in Materials and Methods. Northern blot analysis of Clones 1a (C), 34a (G), and 40b (J). Clones la, 34a, and 40b, each of which was one of the clones from #1, #34, and #40, respectively, were isolated from the plasmids and used as probes for the Northern blot analysis. Expression of #1 (D) and #40 (K) during the cell cycle. Fragment #1 or #40 was labeled and probed on the time course membranes as described in Materials and Methods. SS, 85 hr serum-starved cells; SS+mimo, 85 hr serum starvation + 24 hr 400 µM mimosine treatment in complete medium; SS+mimo+C, 85 hr serum starvation + 24 hr 400 µM mimosine treatment in complete medium + 4 hr complete medium without mimosine.