Fig. 3

Identification and expression of cDNA fragments #11, #12, and #15, which are differentially expressed in G0 verse late G1

Time course samples were used to obtain G0 and late G1 cells (Set 2). Differentially expressed patterns of #11 and #12 (A), and #15 (B) on the DD gel. Differential display method was performed on RNA samples collected following serum induction at 0, 8, 10, and 12 hr as described in Materials and Methods. After fragments #11 (C), #12 (E), and #15 (G) were cut from DD gel and PCR amplification with the corresponding primers used in DD, Northern blot analysis was performed with the time course membranes as described in Materials and Methods. Clones 11c (D), 12b (F), and 15d (H), each of which was one of the clones from #11, #12, and #15, respectively, were isolated from the plasmids and used as probes for the Northern blot analysis.