Fig. 8
From: Engagement of Tumor Necrosis Factor mRNA by an Endotoxin-Inducible Cytoplasmic Protein
Kinetics (A) and LPS dose dependence (B) of complex 2 formation
(A) Upper panel: S100 cytoplasmic extracts were prepared from RAW cells stimulated by LPS (10 ng/ml) for 0, 0.5, 1 or 2 hr and analysed by EMSA using the 3′ TNF AU+ probe. Lower panel: measurement of TNF secreted by the cell cultures used for cytoplasmic extract preparation. (B) Upper panel: EMSA in which S100 cytoplasmic extracts from RAW264.7 macrophages treated for 2 hr with increasing doses of LPS were incubated with labeled 3′ TNF AU+ probe. LPS doses are indicated at the bottom of the figure. Lower panel: measurement of TNF produced by RAW264.7 macrophage cultures used for extract preparation.