Fig. 2

Time course and concentration dependence of IFNγ-induced cell surface ICAM-1 expression on T84 cells

(A) As described in Materials and Methods, T84 cells cultured at 3/4 confluent density on 96-well tissue culture plates were exposed to IFNγ (1000 U/ml) for the times indicated and subsequently assayed for surface ICAM-1 expression using mAb R6.5 by enzyme-linked immunosorbent assay (ELISA). (B) T84 cells similarly prepared were exposed to IFNγ for 48 hr at the concentrations indicated and subsequently assayed for surface ICAM-1 by ELISA as in Panel A. Color was developed and quantitated at OD 405 using a microtiter plate reader as described in Materials and Methods. Optical density at 405 nm represents antibody-treated well values after subtraction of the values from wells treated with irrelevant nonbinding antibody control. Each value represents the mean ± SD of four individual monolayers.