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Fig. 6 | Molecular Medicine

Fig. 6

From: Expression and Polarization of Intercellular Adhesion Molecule-1 on Human Intestinal Epithelia: Consequences for CD11b/CD18-Mediated Interactions with Neutrophils

Fig. 6

Effect of anti-ICAM-1 mAbs on physiologically directed (basolateral-to-apical) PMN transepithelial migration

Transmigration assays were performed as in Fig. 6 except that inverted T84 monolayers were used and antibodies were applied to both apical and basolateral surfaces. As described in Materials and Methods, transmigration assays with such inverted monolayers allow gravitational settling of PMN onto the filter and subsequently, contact with the basolateral aspect of the monolayers prior to initiation of migration. Transmigration is normalized as a percentage of transmigration in the presence of the noninhibitory control antibody W6/32. As can be seen, there is no inhibitory effect of any of the ICAM-1 antibodies in unstimulated (A) or IFNγ-activated (B) monolayers. In addition, transmigration in the absence of antibody (no Ab; A) was not statistically different than that in the presence of W6/32. In data not shown, anti-CD11b (44a) but not anti-CD11a (TS1/22) markedly inhibits (>65%) transmigration in such assays, as previously reported (16,18). Data represents the mean ± SD of four monolayers for each condition; one of three experiments.

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