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Fig. 8 | Molecular Medicine

Fig. 8

From: Expression and Polarization of Intercellular Adhesion Molecule-1 on Human Intestinal Epithelia: Consequences for CD11b/CD18-Mediated Interactions with Neutrophils

Fig. 8

ICAM-1-INDEPENDENT ADHERENCE OF NATURAL HUMAN COLONOCYTES TO PURIFIED CD11B/CD18

Natural human colonocytes obtained from a surgical resection specimen of a patient with severe inflammatory bowel disease were labeled and used in adhesion assays with purified CD11b/CD18 as described in Fig. 7 and in Materials and Methods. As described in the previous figure, the negative antibody control (Ab Control) represents adhesion after treatment of wells with OKM1. As a functionally inhibitory antibody to CD11b, mAb CBRM1/29 was used (Anti-CD11b). Pretreatment of T84 cells with saturating concentrations of functionally inhibitory anti-ICAM-1 antibody R6.5 had no effect on the adhesion to CD11b/CD18. Data represent the mean ± SD of quadruplicate determinations.

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Molecular Medicine

ISSN: 1528-3658