Fig. 3
From: Selection of Novel Analogs of Thalidomide with Enhanced Tumor Necrosis Factor α Inhibitory Activity
The effects of anti-human IL-10 monoclonal antibody (anti-hu IL-10) on the inhibition of TNF α by thalidomide and the analogs in LPS-stimulated monocytes.
Cells were activated with LPS (1 µg/ml) and cultured with drugs in the presence of control IgG or neutralizing anti-huIL-10 (10 µg/ml) for 20 hr. Production of TNFα was determined by ELISA. The ability of anti-huIL-10 to completely neutralize secreted IL-10 was confirmed by ELISA. Results are means ± SD of three experiments done in triplicate. (insert) Kinetics of TNFα and IL-10 release into the culture supernatant after LPS stimulation of human monocytes. Cytokine levels were determined by ELISA.