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Fig. 4 | Molecular Medicine

Fig. 4

From: Abnormal Activation of Glial Cells in the Brains of Prion Protein-deficient Mice Ectopically Expressing Prion Protein-like Protein, PrPLP/Dpl

Fig. 4

A wild-type mouse PrP transgene normalizes the level of glial cell-specific gene expression in the brains of Ngsk Prnp0/0 mice. (a) 10 µg of total RNA extracted from the total brain tissues of 80-week-old Prnp+/+, Ngsk Prnp0/0, and Tg(P) Ngsk Prnp0/0 mice were subjected to Northern blotting using the GFAP and LM probes. The integrity and quantity of RNA were verified with GAPDH probe on the same membrane. (b) The intensities of Northern blot signals shown in (a) were quantified using BAS 5000, the mRNA levels standardized with GAPDH mRNA, and expressed as arbitrary units in comparison with the values of Prnp+/+ mice. Three brains from each line were analyzed by ANOVA. **represents p < 0.01.

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Molecular Medicine

ISSN: 1528-3658