Fig. 5

Glial cell-specific genes are not upregulated in the brains of Zrch I Prnp^0/0 mice lacking Purkinje cell degeneration. (a) The levels of transcripts for GFAP and LM were compared by Northern blotting among total brain tissues from Prnp^+/+ (lane 1), Ngsk Prnp^0/0 (lane 2), Ngsk/Zrch I Prnp^0/0 (lane 3), and Zrch I Prnp^0/0 (lane 4) at 70 weeks of age. 10 µg of total RNA were blotted on each lane, and the integrity and quantity were verified with GAPDH probe on the same membrane. (b) The intensities of Northern blot signals were quantified and plotted as described. The levels of transcripts for GFAP and LM were compared among Prnp^+/+, Ngsk Prnp^0/0, Ngsk/Zrch I Prnp^0/0, and Zrch Prnp^0/0 mice at 70 weeks of age by using arbitrary units and analyzed by ANOVA (n = 3). **represents p < 0.01; *represents p < 0.05.