Fig. 4

Permeation of peroxidase from blood vessels of the cortex after injury. Seven days after cold injury to the cortex, WT (A) and apoE−/− (B) mice were injected i.v. with peroxidase and 6 hours later the intravascular peroxidase was flushed out and brains were processed and stained for peroxidase activity. While almost no positive staining was seen in WT sections, the apoE−/− showed strong deposits of peroxidase periodically distributed along the blood vessels (arrow). A weaker staining could be observed already one hour after a peroxidase infusion in the knockout brains (not shown). Bar = 30 µm.