Fig. 2

Phosphotyrosine immunofluorescence at focal adhesions of MTLn3 Rho family mutant stable lines. Cells were seeded onto 22 mm square collagen-coated glass cover slips and treated with EGF (5 nM) for 4 h. Immunostaining for pTyr and Vinculin were recorded using a cooled CCD camera and the images merged (PY + Vinc). Results are shown for MTLn3-control (A), MTLn3-N17Cdc42 (B), MTLn3-N17Rac1 (C), and MTLn3-N19RhoA (D). Inhibition of pTyr IF is found in (B), and (D).