Fig. 5From: Lack of Plasma Membrane Targeting of a G172D Mutant Thiamine Transporter Derived from Rogers Syndrome FamilyCellular localization of an N-glycosylation deficient N63Q mutant THTR-1, N63Q. (A) Swiss 3T3 cells were cotransfected with both GFP and THTR-1 N63Q mutant expression plasmids. Transfected cells were fixed and stained with anti-Myc 9E11 antibodies. The localization of GFP alone (green, panel A) or CY3-conjugated goat anti-mouse antibody staining alone (red, panel B) and costaining (panel C) is shown. Yellow stain indicates colocalization of the green and red fluorescence.Back to article page