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Fig. 5 | Molecular Medicine

Fig. 5

From: Lack of Plasma Membrane Targeting of a G172D Mutant Thiamine Transporter Derived from Rogers Syndrome Family

Fig. 5

Cellular localization of an N-glycosylation deficient N63Q mutant THTR-1, N63Q. (A) Swiss 3T3 cells were cotransfected with both GFP and THTR-1 N63Q mutant expression plasmids. Transfected cells were fixed and stained with anti-Myc 9E11 antibodies. The localization of GFP alone (green, panel A) or CY3-conjugated goat anti-mouse antibody staining alone (red, panel B) and costaining (panel C) is shown. Yellow stain indicates colocalization of the green and red fluorescence.

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