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Fig. 2 | Molecular Medicine

Fig. 2

From: α-Secretase ADAM10 as Well as αAPPs Is Reduced in Platelets and CSF of Alzheimer Disease Patients

Fig. 2

WB analysis of ADAM10 in human cortex and human platelets. (A) Representative WB analysis of ADAM10 in whole platelet homogenate of a control subject. Increasing protein concentrations were loaded on gel (8% SDS-PAGE). The arrow shows the migration of the 68-kDa ADAM10 immunoreactive band. No other bands are present at higher MW. No unspecific signal was obtained by incubating nitrocellulose with the secondary antibody alone (rightmost lane). Molecular weight markers are reported on the right. (B) Left panel: Representative WB analysis of ADAM10 in human cortical homogenate of a control subject. Fifty micrograms of proteins were loaded on 8% acrylamide gel. Molecular weight markers are reported on the right. Right panel: Representative WB analysis of ADAM10 in human cortex after immunoprecipitation: 50 µg of human cortex proteins were incubated in presence (+) and in absence (−) of pAb anti-ADAM10 in RIA buffer and then precipitated with protein A-agarose beads. The immunoprecipitated samples were then loaded into 8% SDS-PAGE and recognized with pAb anti-ADAM10. Two forms are present (arrowheads), enriched in the immunocomplex in comparison with WB analysis (left panel). A major band is present at 85 kDa, corresponding to the precursor protein, and a second band at 68 kDa, corresponding to the mature form of the protein. They represent two specific forms because they are not detected when the total protein was incubated in the absence of antibody (−). Molecular weight markers are reported on the right.

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