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Fig. 5 | Molecular Medicine

Fig. 5

From: α-Secretase ADAM10 as Well as αAPPs Is Reduced in Platelets and CSF of Alzheimer Disease Patients

Fig. 5

Quantitative analysis of α APPs in platelets and CSF of control subjects and AD patients. (A) Representative Western blot analysis performed on supernatants of unstimulated and activated platelets from AD patients and control subjects with mAb 6E10, recognizing αAPPs. Total proteins released from resting (−) platelets and from platelets activated with thrombin (+) were desalted and loaded into a 6% acrylamide gel. A 130-kDa form immunoreactive for 6E10 is present (arrowhead). Apparent molecular weight is reported on the right. (B) Quantitative analysis of 6E10 Western blot from platelet supernatants are reported (control, n = 12; AD, n = 15). Immunoreactive bands were measured by computer-assisted imaging. A significant reduction in the levels of 130-kDa form was observed in AD patients (p < 0.001).

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