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Ubiquitous Induction of p53 in Tumor Cells by Antisense Inhibition of MDM2 Expression

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Abstract

Background

The MDM2 oncogene functions as a negative feedback regulator of the p53 tumor suppressor. Abnormal expression of MDM2 in tumors may attenuate the p53-mediated growth arrest and apoptosis response, resulting in increased cell proliferation and resistance to chemotherapy.

Materials and Methods

We have developed phosphorothioate antisense oligodeoxynucleotides optimized for inhibition of MDM2 expression and investigated the role of MDM2 in a large panel of tumor cell lines.

Results

Inhibition of MDM2 expression in 15 tumor types containing wild-type p53 results in a significant induction of nuclear p53 accumulation. The increase in p53 level is due to prolonged half-life and is associated with an increase in p53 transcriptional activity, growth inhibition, or apoptosis. Inhibition of MDM2 expression is also sufficient to induce nuclear p53 accumulation in several cell lines with cytoplasmic p53.

Conclusions

The MDM2 negative feedback loop is important for maintenance of p53 at a low level by promoting p53 degradation. Nuclear export and degradation by MDM2 may contribute to the p53 nuclear exclusion phenotype. Inhibition of MDM2 expression can effectively activate p53 in most tumor types, including those without MDM2 overexpression, and may have broad anti-tumor potential.

Introduction

The p53 tumor suppressor gene functions through induction of growth arrest or apoptosis in response to stress or genomic damage (1). About 50% of tumors carry p53 mutations (2), suggesting that presence of a functional p53 pathway is incompatible with tumor growth. Tumors without p53 mutation may contain an impaired p53 pathway due to alternative mechanisms, such as overexpression of the MDM2 oncogene (3,4). p53 is an important mediator for the anti-cancer effects of many chemotherapeutic agents and ionizing irradiation (5). Therefore, development of methods to activate p53 in tumor cells will shed light on the mechanism of p53 regulation and provide a potential new anticancer strategy.

The MDM2 oncogene was first cloned as an amplified gene on a murine double-minute chromosome (6). Overexpression of MDM2 in cells increases the tumorigenic potential (6) and can cooperate with an activated ras oncogene to attain full transformation (7). The MDM2 gene is amplified or overexpressed in about 40–60% of human osteogenic sarcomas and about 30% of soft-tissue sarcomas (3,4), implicating its role in the development of these malignancies.

An important function of MDM2 is to bind to and inhibit the p53 tumor suppressor (8). MDM2 can also promote degradation of p53 through a ubiquitin-dependent proteolytic pathway by acting as a ubiquitin ligase E3 (911). p53 activates MDM2 expression at the level of transcription (12,13), suggesting that MDM2 can function as a negative feedback regulator of p53. Experiments using MDM2 knockout mice confirmed that an important function of MDM2 is to negatively regulate p53 (14,15). Recently, it was demonstrated that introduction of antibody 3G5 (16), or an engineered protein that can inhibit MDM2-p53 binding, resulted in stimulation of p53 activity and accumulation of p53 in MCF-7 and T22 cells (17). Therefore, MDM2 may have a constitutive role in promoting p53 turnover.

We previously identified a phosphorothioate antisense oligonucleotide against human MDM2. Inhibition of MDM2 expression in a tumor cell line (JAR) with MDM2 amplification resulted in activation of p53 and apoptosis. The MDM2 antisense oligonucleotide also enhanced the ability of a DNA damaging agent (camptothecin) to induce p53 in a synergistic fashion (18), suggesting that the MDM2 feedback loop may limit the magnitude of p53 activation during a DNA-damage response. Therefore, inhibition of MDM2 expression may have therapeutic potential against human tumors expressing wild-type p53. However, many questions remain to be addressed, i.e., the effects of inhibiting MDM2 expression in tumors with different p53 mutation status, level, localization, stability, and MDM2 level. Furthermore, oligonucleotides identified in a primary screen is unlikely to be optimal.

In this study, we have developed a significantly improved MDM2 antisense phosphorothioate oligodeoxynucleotide that is a potent activator of p53. Inhibition of MDM2 expression in a large panel of human tumor cell lines using this oligonucleotide revealed that MDM2 is widely involved in suppressing the accumulation of p53. Therefore, inhibition of MDM2 expression is a powerful method for activating p53 by inducing p53 accumulation in the absence of DNA damage.

Materials and Methods

Synthesis of Oligodeoxynucleotides

Phosphorothioate oligodeoxynucleotides were synthesized using β-cyanoethyl phosphoramidite chemistry on an automated synthesizer (Expedite 8909, Perceptive Biosystems, Framingham, MA) and purified by preparative reverse-phase high-performance liquid chromatography. Seven 20-mer phosphorothioate antisense oligodeoxynucleotides (AS1–AS7) were synthesized for this screen (Fig. 1). A 2-bp mismatch control (AS2M2: 5′TGACACTTGTTCTTACTCAC) and a 4-bp mismatch control oligodeoxynucleotide (AS2M4: 5′TGACTCTTGTCCTTACTCAC) for AS2 were also synthesized. The oligodeoxynucleotide K (5′CAGAGCCTTCATCTTCCCAG) is a control against an unrelated target.

Fig. 1
figure1

Characterization of MDM2 antisense oligonucleotides.

(A) Sequence of MDM2 antisense phosphorothioate oligonucleotides. The top sequence represents the noncoding strand of human MDM2. The numbers indicate distance from the translation initiation codon. (B) Screening of MDM2 antisense oligonucleotides. MCF-7 cells stably transfected with the BP100-luc reporter were treated with 50 nM of MDM2 oligonucleotides for 20 hr. p53 transcriptional activity was determined as luciferase activity/unit protein. M4 is a 4-bp mismatch control of AS. LC, control with lipofection alone. (C) Activation of p53 by AS2 is sequence-specific. JAR cells stably transfected with the BP100-luc reporter were treated with AS2, mismatch control oligonucleotides of AS2 (AS2M2, 2-bp mismatch; AS2M4, 4-bp mismatch), and an unrelated oligonucleotide K. (D) Inhibition of MDM2 expression by AS2. MDM2 levels in JAR cells treated for 20 hr with AS2 or AS2M4 control oligonucleotide were determined by Western blotting using monoclonal antibody 3G9. JAR cells treated with lipofectin alone, loaded at 1-, 2-, and 4-fold dilutions, were used as controls.

Cell Lines

JAR, JEG-3, SJSA, MCF-7, U87-MG, SK-N-SH, U20S, Caski, C33A, DLD-1, and A549 cells were obtained from the ATCC. WI-38, JeKin, HepG2, LS180, HT1080, G361, PA-1, and Lncap cells were obtained from the cell culture core lab of LSU Medical Center. H1299, MCF-7, MDA-MB-231, and Hela cells were from Dr. Arnold J. Levine (Princeton University). The 101 cell line was provided by Dr. James Gnarra (LSU Medical Center). The SLK cell line was kindly provided by Dr. Om Prakash (Ochsner Foundation). All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS).

Antisense Oligonucleotide Treatment

Cells were cultured in DMEM medium with 10% FBS. Cell lines normally grown in other types of medium were also adapted to growth in DMEM with 10% FBS before the treatment. Before addition of oligonucleotides, cells were refed with DMEM containing 1% FBS. Lipofectin (Gibco BRL) was incubated with serum-free DMEM medium for 45 min, then mixed with the oligonucleotides for 10 min and added to the cell culture. The final concentration of Lipofectin was 7 µg/ml, final concentration of FBS was 0.75%. Cells were incubated with oligonucleotides and Lipofectin for 18–24 hr as indicated.

Western Blot

Cells were lysed in RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 1% Na deoxycholate) and 100 µg of the protein lysate were fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon P filters (Millipore). The filters were blocked for 5 min with 5% non-fat dry milk, incubated with anti-p53 monoclonal antibody DO-1 or an anti-MDM2 monoclonal antibody 3G9 (19), then incubated with protein A-peroxidase (Promega), washed, and developed using the ECL-plus reagent (Amersham). All incubations were carried out in phosphate-buffered saline (PBS) with 5% non-fat milk and 0.1% Tween-20.

Stable Transfection of Cell Lines

Cells were co-transfected with the BP100-luciferase reporter plasmid or pActin-E6 plasmid and a G418-resistant marker plasmid pCMV-neo-Bam using the calcium-phosphate precipitation method. Transfected cells were grown in medium with 750 µg/ml G418 until colonies appeared. Individual colonies were isolated and expanded into cell lines. The JAR-BP100-luc is a clonal cell line isolated by diluting a pool of BP100-luciferase-transfected JAR cells from a previous experiment (18).

Immunofluorescence Staining

For p53 staining, cells were treated with oligonucleotides for 20 hr, fixed with acetone-methanol (1:1) for 3 min, then blocked with PBS+10% normal goat serum (NGS) for 20 min, and incubated with Pab1801 hybridoma supernatant for 2 hr. To stain for MDM2, cells were fixed in 4% paraformaldehyde in PBS for 20 min, blocked with PBS+10% NGS for 20 min, and incubated with 2A9 hybridoma supernatant at 1/100 dilution in PBS+10% NGS for 2 hr. Slides were washed with PBS+0.1% Triton X-100, incubated with FITC-goat-anti-mouse IgG in PBS+10% NGS for 1 hr, washed with PBS+0.1% TritonX-100, and mounted.

Determination of p53 Half-Life

SJSA cells were treated with 200 nM AS2 for 20 hr. Cells were incubated with DMEM (without methionine) with 2% dialyzed FBS, 50 uCi/ml 35S-EXPRESS (NEN) for 2 hr and refed with regular medium. Sample plates were collected at indicated time points and lysed with lysis buffer (50 mM Tris, pH 8.0, 5 mM EDTA, 150 mM NaCl, 0.5% NP40, 1 mM PMSF). Cell lysates with identical levels of radioactivity (2 × 107 CPM) were immunoprecipitated with Pab421 and Pab1801, washed with 50% SNNTE buffer (25 mM Tris, pH 7.4, 2.5 mM EDTA 2.5% sucrose, 1% NP-40, 250 mM NaCl), and fractionated by SDS-PAGE. P53 was detected by autoradiography.

Determination of Cell Proliferation Rate

Cells were treated with 100 nM of oligonucleotides for 20 hr, labeled with BrdU for 2 hr, and incubated with MTS reagent (Promega) for 1 hr. Relative cell viability was determined by measuring OD at 490 nm (reduction of MTS substrate by mitochondria activity). Cells were then fixed and the level of BrdU incorporation was determined using a chemiluminscence ELISA assay (Boehringer Mannheim). The rate of DNA synthesis was determined as BrdU incorporation/OD 490.

Results

Optimization of MDM2 Antisense Oligonucleotide

In a previous study, a screen using nine oligonucleotides identified HDMAS5 (“AS” below) to be the most effective in inhibition of MDM2 expression in JAR cells (18). Because the AS oligonucleotide may have revealed a region of the MDM2 mRNA that is sensitive to antisense inhibition, a second screen was performed with seven oligonucleotides adjacent to AS (Fig. 1A). Oligonucleotides were tested for activation of p53 transcription function using JAR cells (over-expressing MDM2 due to gene amplification) and MCF-7 cells (expressing lower levels of MDM2) stably transfected with the p53-responsive reporter plasmid BP100-luciferase.

The results showed that several of the secondary oligonucleotides were more effective than AS in causing activation of p53 (Fig. 1B). An oligonucleotide AS2 was the most potent of this group and was chosen for further characterization. AS2 was 5-fold more efficient than AS in MCF-7 cells (Fig. 1B) and 2-fold more efficient than AS in JAR cells (not shown) at a concentration of 50 nM. The effect of AS2 is sequence-specific. Introduction of two or four nucleotide mismatches into the sequence significantly inhibited its ability to activate p53 (Fig. 1C). Treatment of JAR cells with AS2 inhibited MDM2 protein expression in a sequence-dependent and dose-dependent fashion (Fig. 1D), suggesting that its ability to activate p53 is due to specific inhibition of MDM2 expression. Consistent with its increased ability to activate p53, AS2 is more efficient in inhibiting MDM2 expression (IC50: 25 nM, Fig. 1D) than AS (IC50: 100–200 nM) (18) in JAR cells. When AS2 or AS (20 nucleotides) were shortened to 18 nucleotides from one or both ends, the ability to activate p53 was also significantly reduced (not shown).

Apoptotic Function of AS2 Is p53-Dependent

Similar to the parent AS oligonucleotide, AS2 also induces apoptosis in JAR cells. To further delineate whether AS2 induces apoptosis through activation of p53, a JAR cell line expressing the E6 oncogene of HPV16 was created (JAR-E6). Expression of E6 under the actin promoter results in degradation of p53, as demonstrated by the loss of p53 protein in a Western blot (Fig. 2). Interestingly, the level of MDM2 expression also decreases significantly in JAR-E6 cells, suggesting that in addition to gene amplification, activation by p53 is an important mechanism of MDM2 overexpression in this cell line.

Fig. 2
figure2

Induction of apoptosis by AS2 is p53-dependent.

(A) JAR cells stably transfected with an actin promoter-driven HPV E6 construct (JAR-E6) expressed no detectable p53 and significantly reduced level of MDM2 in Western blot analyses. Identical amounts of total protein were loaded on each lane. (B) JAR-E6 cells are resistant to apoptosis induction by AS2. JAR and JAR-E6 cells were treated with 200 nM of AS2 for 24 hr. AS2 induced significant cell death in JAR cells, but not in JAR-E6 cells.

When treated with 200 nM of AS2, which efficiently induced apoptosis in parental JAR cells, JAR-E6 cells showed little apoptosis (Fig. 2). This result suggests that AS2 induces apoptosis through specific activation of p53. The JAR-E6 cells are not resistant to apoptosis in general; treatment with DNA-damaging agents (camptothecin, etoposide) at concentrations that can kill other p53-null cells also induced cell death in JAR-E6 (not shown).

Induction of p53 Accumulation by Inhibition of MDM2 in Different Cell Lines

The strong activation of p53 in MCF-7 cells by AS2 (Fig. 1) prompted us to further examine its effect on p53. MCF-7 cells predominantly contain a cytoplasmic form of p53 (20) and displayed a predominantly cytoplasmic fluorescence when stained using anti-p53 monoclonal antibody Pab1801. After treatment with 200 nM AS2 for 20 hr, many MCF-7 cells show intense nuclear p53 staining (Fig. 3). The parent AS oligonucleotide also showed a similar but weaker ability to induce p53 accumulation (not shown), and the control oligonucleotide K did not induce p53 (Fig. 3). This suggests that MDM2 plays an important role in inhibiting the accumulation of nuclear p53 in this cell line. Because there is an increase in total p53 after AS2 treatment (see below in Fig. 4), and there is not an obvious loss of cytoplasmic p53 staining in cells accumulating nuclear p53, the results suggest that MDM2 normally promotes the degradation of nuclear p53 in MCF-7 cells.

Fig. 3
figure3

Induction of p53 accumulation by AS2 in different tumor cell lines.

Cells were cultured on chamber slides, treated with 200 nM AS2 or control oligonucleotide K for 20 hr, and stained for p53 expression using Pab1801. Treatment with AS2 induced strong nuclear p53 accumulation in cells with low basal levels of wild-type p53.

Fig. 4
figure4

Stabilization of p53 by inhibition of MDM2 expression.

(A) Inhibition of MDM2 expression results in an increase of p53 level. Cells were treated with 200 nM of AS2 or control oligonucleotide K for 20 hr. p53 protein levels were detected by Western blot with antibody DO-1. Identical amounts of total protein were loaded on each lane. The double band in MCF-7 is due to a p53 polymorphic allele. (B) Determination of p53 half-life. SJSA cells were treated with 200 nM AS2 for 20 hr and the rate of p53 degradation was determined by a pulse-chase experiment. The half-life of p53 was 0.5 hr in untreated SJSA cells and >4 hr in AS2 treated cells as determined by densitometric analysis. The total CPM used for untreated samples was 2-fold higher than for AS2-treated samples, thus the p53 band in 0 hr untreated sample is darker and does not indicate a different rate of synthesis.

To determine whether MDM2 also exhibits a similar role in other tumors containing cytoplasmic p53, the neuroblastoma cell line SK-N-SH was tested. Neuroblastomas rarely have p53 mutations but often contain p53 in the cytoplasm. SK-N-SH cells express cytoplasmic wild-type p53 and exhibit a reduced ability to undergo cell cycle arrest after DNA damage (21,22). When treated with AS2, this cell line also displayed a strong accumulation of nuclear p53 in nearly 100% of the cells, as well as an increase in total p53 level (not shown). This result suggests that in addition to cytoplasmic sequestration, MDM2-mediated degradation may be an important mechanism for the loss of nuclear p53 in some tumors (also see Discussion).

This test was then extended to a wide variety of tumor cell lines with wild-type p53. A total of 24 human tumor cell lines representing 15 different tumor types were treated with AS2 and stained for p53 expression. The results, as shown in Figure 3 and summarized in Table 1, revealed that the low levels of wild-type p53 can be significantly stimulated by AS2, resulting in intense nuclear p53 staining. Two nontransformed human cell lines, WI-38 (lung fibroblast) and JeKin (skin fibroblast), also showed strong p53 accumulation after inhibition of MDM2 expression. Therefore, this p53 response to inhibition of MDM2 expression is not unique to tumor cells.

Table 1 Induction of p53 accumulation by inhibition of mdm2 expression a

Treatment of tumor cells with homozygous mutant p53 also did not lead to further accumulation of p53, which was already at a high level. A tumor cell line with both wild-type and mutant p53 alleles (PA-1) also contains inducible p53 and underwent apoptosis after AS2 treatment (see below). Finally, treatment of HPV-positive cervical cancer cell lines did not induce p53 accumulation, suggesting that HPV E6-mediated degradation of p53 is independent of MDM2 function.

Inhibition of MDM2 Expression Prolonged p53 Half-Life

The increase in p53 after inhibition of MDM2 expression can result from an increased rate of p53 synthesis or protein stabilization. To directly test these possibilities, the p53 half-life in AS2-treated SJSA cells was determined by a pulse-chase radioactive labeling experiment. SJSA cells have MDM2 gene amplification and exhibit a highly inducible wild-type p53 after AS2 treatment (Figs. 3, 4A). This cell line does not undergo significant apoptosis after AS2 treatment, thus it can provide sufficient material for analysis.

SJSA cells were treated with 200 nM AS2 for 20 hr and pulse labeled with 35S-methionine for 2 hr. The level of p53 was determined at various times after addition of excess cold methionine to prevent further synthesis of radioactive p53. The result showed that the half-life of p53 is increased from 0.5 hr in untreated SJSA cells to >4 hr in AS2-treated cells. Furthermore, the amount of radioactive MDM2 synthesized during the 2-hr pulse-labeling period did not differ significantly in treated and untreated cells, as revealed by the levels of MDM2 at time zero of the chase (Figure 4B). Therefore, the rise in p53 level after inhibition of MDM2 expression appears to be due to the stabilization of p53, and not to its increased synthesis.

Inhibition of MDM2 Expression Induced Functional p53

To further determine whether the p53 protein that accumulates after inhibition of MDM2 is functionally active, a p53-responsive BP100-luciferase reporter plasmid was transfected into several representative cell lines. Stably transfected cells were then treated with AS2 or control oligonucleotides. The results showed that a strong induction of p53 transcription function occurs after inhibition of MDM2, demonstrating that the p53 accumulated after inhibition of MDM2 is highly active (Fig. 5). The magnitude of p53 transcription activation is consistent with the fact that most of the p53 accumulation occurs in the nucleus, which has a low basal level of p53. The 4-bp mismatch control oligonucleotide has significantly reduced efficiency in activation of p53, particularly at low concentrations.

Fig. 5
figure5

Induction of p53 transcriptional activation function.

Cells stably transfected with the BP100-luciferase reporter were treated with antisense oligonucleotides at indicated concentrations for 20 hr. Luciferase activity/unit protein was determined and the magnitude of induction was shown, compared to cells not treated with oligonucleotides. AS2M4, a 4-bp mismatch control of AS2; K, an unrelated oligonucleotide.

Inhibition of MDM2 Leads to Growth Arrest and Apoptosis

After a 20-hr treatment with 200 nM AS2, a significant amount of cell death was evident in some of the tumor cell lines tested (HT1080, PA-1, SK-N-SH; Fig. 6). In these cases, cells rounded up, displayed membrane ruffling and blebbing characteristic of apoptosis, and detached from the culture surface. Staining of nuclear DNA also showed shrinkage and fragmentation of nuclei (Fig. 6B). Thus, it appears that the level of p53 activation achieved by treatment with AS2 can be sufficient to induce cell death through apoptosis in some cell lines examined.

Fig. 6
figure6

Induction of cell death by inhibition of MDM2 expression.

Cells were treated with 200 nM AS2 or control oligonucleotide K for 24 hr and photographed. (A) Examples of cell lines undergoing significant cell death characteristic of apoptosis are shown. (B) SK-N-SH cells treated with AS2 were stained with the DNA dye DAPI to reveal nuclear fragmentation and shrinkage of apoptotic cells.

Some of the tumor cell lines (MCF-7, SJSA, A549, A174) as well as two nontransformed cell lines (WI-38 and Jekin) showed little cell death after a 20-hr AS2 treatment. Since p53 activation can lead to apoptosis or cell cycle arrest, dependent on the level of p53 and the status of the cell, several of these cell lines were further tested for growth arrest by AS2. Cells were treated with AS2 or control oligonucleotides for 20 hr, and DNA synthesis was quantitated by BrdU incorporation. The number of viable cells was determined by incubation with the MTS reagent. The results show that in cell lines that do not undergo significant apoptosis after AS2 treatment, the rate of DNA synthesis is reduced (Fig. 7). This effect is weaker with a 4-bp mismatch control oligonucleotide and is not observed with the unrelated oligonucleotide K. In contrast, the p53-null cell lines H1299 (human) and 10(1) (mouse) (23) did not undergo significant growth inhibition. Therefore induction of p53 by inhibition of MDM2 can lead to growth arrest or apoptosis.

Fig. 7
figure7

Inhibition of DNA synthesis by MDM2 antisense oligonucleotides.

Cells were treated with 100 nM of oligonucleotides for 20 hr and labeled for 2 hr with BrdU. Incorporation rate of BrdU was determined by an ELISA assay and normalized to the number of viable cells. H1299 and 10(1) cells are human and mouse cells devoid of p53. LC, lipofectin treatment alone.

MDM2-Mediated Degradation of p53 Is Inefficient in Two Choriocarcinoma Cell Lines

The results presented in Table 1 also reproduce our previous observation that in JAR and JEG-3 cells with high levels of p53 and MDM2, inhibition of MDM2 expression did not result in a significant increase in p53 level. These notable exceptions suggest that the ability of MDM2 to promote p53 degradation may be suppressed in choriocarcinoma cell lines, which predict that p53 is not actively degraded through the ubiquitin-dependent proteasome pathway in these cells.

To test this possibility, JAR and JEG-3 cells were treated with the proteasome inhibitor MG132, which blocks ubiquitin-dependent degradation of proteins (24). PA-1 cells that have highly inducible p53 after MDM2 antisense treatment were used as a control. Treatment with 10 µM of MG132 for 3–6 hr resulted in an over 10-fold increase in MDM2 in all three cell lines (Fig. 8), confirming a previous report that MDM2 is degraded through the ubiquitin-dependent proteasome pathway (25). MG132 only induced a weak (<2-fold) increase of p53 in JAR cells and JEG-3 cells (Fig. 8). In contrast, PA-1 cells showed an over 5-fold increase in p53 level after MG132 treatment. These results confirm that p53 is not efficiently degraded by the ubiquitin-dependent proteasome pathway in JAR and JEG-3 cells, consistent with the fact that they have stabilized wild-type p53 in the presence of high levels of MDM2 (26).

Fig. 8
figure8

Effects of proteasome inhibitor MG132 on p53 and MDM2 levels.

Cells were treated with 10 µM MG132 for 3 or 6 hr. Identical amounts of total cellular protein were fractionated by SDS-PAGE and analyzed by Western blot with monoclonal antibody 3G9 for MDM2 levels, and DO-1 for p53 levels.

Discussion

In this study, we have demonstrated that antisense inhibition of MDM2 expression activates p53 in tumor cells containing either low or high levels of MDM2. Importantly, inhibition of MDM2 expression in cells with low levels of p53 uniformly results in p53 accumulation and increase in p53 activity. This response occurred in nearly all of the tumor and nontransformed cells tested. The only exceptions were HPV-positive cells, which have an E6 oncogene-mediated mechanism of p53 degradation. The accumulation of p53 is due to a prolonged half-life, not an increased rate of synthesis. These results suggest that MDM2 expression is important for maintaining p53 at low levels. The results also suggest that in addition to possibly playing a role in post-stress down-regulation of p53 (9,10,27), the p53-degradation function of MDM2 is constitutively active in tumor cells in non-stress conditions.

Induction of DNA strand breaks by a variety of methods have been shown to cause p53 accumulation (28,29), probably by modulation of p53-MDM2 binding (27). The control experiments we performed suggest that MDM2 antisense oligonucleotide induces strong p53 accumulation mainly through a target-specific antisense mechanism. The activation of p53 by AS2 is highly dependent on a perfect sequence match with the target, and correlates with inhibition of MDM2 expression. A control oligonucleotide with no sequence relationship to MDM2 only weakly activates p53 (2-fold) at concentrations above 300 nM. Therefore, the nonspecific effect of oligonucleotides on p53 is negligible at the dosages we used.

Our observations suggest that MDM2 overexpression is not the only indicator for p53 being in a functionally suppressed state. In tumor cells with low levels of MDM2 (which usually correlates with low levels of wild-type p53), MDM2 is also important for inhibiting p53 accumulation. It is possible that the p53-suppression efficiency of the MDM2 feedback loop has increased during tumor development, such as due to p53-independent induction of MDM2 expression (30). Thus, MDM2 can play an active role in tumor development in the absence of overexpression because of imbalance of the p53-MDM2 regulatory loop. Alternatively, having low levels of wild-type p53 and MDM2 in a tumor may indicate that the MDM2 regulatory loop is not reacting to tumor development because of defects in the signal transduction pathway, or that certain types of oncogenic changes do not trigger a p53 response.

Cytoplasmic accumulation of wild-type p53 is frequently observed in breast tumors and neuroblastomas (21,31), and may represent an alternative mechanism of p53 inactivation. A model of cytoplasmic p53 sequestration through inhibition of nuclear translocation has been proposed to explain this phenomenon (32). Our results with MCF-7 and SK-N-SH cells suggest that MDM2 plays an important role in preventing nuclear p53 accumulation in these cells. Inhibition of MDM2 expression can lead to accumulation of nuclear p53 and increase in total cellular p53. Recent findings suggest that MDM2 can promote p53 degradation (9,10), and this activity is dependent on the nuclear export function of MDM2 (33). Collectively, these results suggest that the cytoplasmic p53 phenotype reflects a dynamic process in which p53 enters the nucleus, binds to MDM2, exits the nucleus as a p53-MDM2 complex, and is degraded or sequestered into cytoplasmic particles. Other experimental approaches are needed to further elucidate this phenomenon.

Two choriocarcinoma cell lines (JAR, JEG-3) show an interesting deviation from most cell lines. Consistent with the observation that they have high levels of MDM2 and stable wild-type p53 (26), inhibition of MDM2 expression or inhibition of proteasome function do not lead to significant accumulation of p53. These results are consistent with the notion that in JAR and JEG-3 cells, MDM2 inhibits p53 mainly through formation of inactive complexes, not promotion of p53 turnover. Whether this is characteristic of trophoblastic tumors and reflects a defect (e.g., lack of a co-factor, point mutation in MDM2) or an active regulation of this degradation pathway remains to be investigated.

Our results demonstrate that inhibition of MDM2 expression is a highly efficient method of activating p53 without causing DNA damage, and may have therapeutic potential against a wide range of tumors. However, systemic inhibition of MDM2 expression will also activate p53 in normal cells that are undergoing rapid proliferation, such as hematopoietic cells and mucosal epithelial cells. These cells are sensitive to p53-induced apoptosis after DNA damaging treatments, as indicated by experiments using p53 knockout mice (3437). Therefore, the anti-tumor specificity and general toxicity of MDM2 inhibitors require further study.

Inhibition of MDM2 activates p53 uniformly and much more efficiently than many DNA-damaging chemotherapy agents (camptothecin, adriamycin, cisplatin, etoposide, fluorouracil, and methotrexate, unpublished results, J. Chen). The ability of these drugs to activate p53 is dependent on cellular uptake, target sensitivity, and the integrity of signal transduction pathways to p53 in individual tumors. Inhibition of MDM2 expression can bypass these deficiencies and achieve strong p53 activation in a broad range of tumor types; thus it may be useful to augment drugs that are inefficient in activating p53 and complement drugs that do not activate p53.

The results of this study suggest that MDM2 is a useful drug target in many tumor types, even when it is not a causative factor during tumor development. Many types of tumors have frequencies of 20–30% (2). Therefore, inhibitors of MDM2 may be useful for the majority of such cases. With the recent approval of the first antisense oligonucleotide-based drug, Fomivirsen, for the treatment of CMV retinitis in AIDS patients, it is evident that this type of compound also has a therapeutic potential. The AS2 oligonucleotide provides a starting point for chemical improvements and further investigation of its anti-tumor potential.

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Acknowledgments

We are particularly grateful to Dr. Jay Hunt for use of the fluorescence microscope. We also thank Joelle Finley, Dr. James Gnarra, and Dr. Om Prakash for providing cell lines, and Dr. Ronald Luftig for critical reading of the manuscript. This work was supported by funding from the Stanley S. Scott Cancer Center and a grant from the American Cancer Society to J. Chen.

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Correspondence to Dr. Jiandong Chen.

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Chen, L., Lu, W., Agrawal, S. et al. Ubiquitous Induction of p53 in Tumor Cells by Antisense Inhibition of MDM2 Expression. Mol Med 5, 21–34 (1999) doi:10.1007/BF03402136

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