Fig. 2
From: Ubiquitous Induction of p53 in Tumor Cells by Antisense Inhibition of MDM2 Expression
Induction of apoptosis by AS2 is p53-dependent.
(A) JAR cells stably transfected with an actin promoter-driven HPV E6 construct (JAR-E6) expressed no detectable p53 and significantly reduced level of MDM2 in Western blot analyses. Identical amounts of total protein were loaded on each lane. (B) JAR-E6 cells are resistant to apoptosis induction by AS2. JAR and JAR-E6 cells were treated with 200 nM of AS2 for 24 hr. AS2 induced significant cell death in JAR cells, but not in JAR-E6 cells.