Molecular Medicine

Table 1 Induction of p53 accumulation by inhibition of mdm2 expression ^a

From: Ubiquitous Induction of p53 in Tumor Cells by Antisense Inhibition of MDM2 Expression

				Nuclear p53 Level
Cell Line	Origin	MDM2 Level	p53	Basal	AS2 Treated
JEG-3	Choriocarcinoma	+ + + +	wt	+ + + +	+ + + +
JAR	Choriocarcinoma	+ + + +	wt	+ + + +	+ + + +
SJSA	Osteosarcoma	+ + + +	wt	−	+ + + +
LS180	Colon carcinoma	−	wt	−	+ + + +
HT1080	Fibrosarcoma	+	wt	+	+ + + +
A172	Glioblastoma	−	wt	−	+ + + +
U87-MG	Glioblastoma	−	wt	+	+ + + +
HepG2	Hepatocarcinoma	+	wt	+	+ + + +
SLK	Kaposi’s sarcoma	−	ND	+	+ + + +
101	Kidney tumor	−	wt	+	+ + + +
A549	Lung tumor	+	wt	+	+ + + +
G361	Melanoma	+ +	wt	+	+ + + +
SK-N-SH	Neuroblastoma	−	wt	−	+ + + +
MCF-7	Breast carcinoma	+	wt	+	+ + + +
U2OS	Osteosarcoma	+	wt	+ +	+ + +
PA-1	Ovarian teratoma	+	wt/mt	−	+ + + +
Lncap	Prostate carcinoma	−	wt	+	+ + + +
WI-38	Lung fibroblast	+	wt	−	+ + + +
JeKin	Skin fibroblast	−	wt	+	+ + + +
MDA-MB-231	Breast carcinoma	+ +	mt	+ + + +	+ + + +
DLD-1	Colon carcinoma	+ +	mt	+ + + +	+ + + +
C33A (HPV−)	Cervical carcinoma	+ +	mt	+ + + +	+ + + +
Hela (HPV+)	Cervical carcinoma	−	wt	−	−
Caski (HPV+)	Cervical carcinoma	−	wt	−	−
H1299	Lung tumor	−	Null	−	−
SK-N-MC	Neuroblastoma	—	Null	—	—

^aCells were treated with 200 nM of AS2 or K oligonucleotide for 20 hr and p53 level was determined semi-quantitatively by immunofluorescence staining with Pab1801. Mdm2 levels in untreated cells were determined by staining with 2A9.
−, not detectable; +, weak staining in most cells or a subset of cells; + +, moderate staining in most cells or a subset of cells; + + + +, strong staining in most or all cells; mt, mutant; ND, not determined; wt, wild type. Cell lines without MDM2 IF signal have been confirmed to express low levels of MDM2 by Western blot.

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ISSN: 1528-3658

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