Fig. 3
From: Two Neutralizing Human Anti-RSV Antibodies: Cloning, Expression, and Characterization
Binding curve of monoclonal antibody RF-1 and cRF-1 (A) and RF-2 and cRF-2 (B) to RSV F-protein.
(A) Purified RSV fusion protein was used as capture in an ELISA to determine binding activity of B cell-produced RF-1 (∇) versus CHO cell-produced cRF-1 (●). Direct binding of a dilution series of the two antibodies was visualized via HRP-labeled secondary antibodies. (B) Purified RSV fusion protein was used as capture in an ELISA to determine binding activity of B cell-produced RF-2 (∇) vs. CHO cell produced cRF-2 (●). Direct binding of a dilution series of the two antibodies was visualized via HRP-labeled secondary antibodies.