Table 1 Synthetic oligonucleotide primers used for PCR amplification of immunoglobulin heavy- and κ light chain variable region genes from cell lines RF-1 and RF-2^a

5′ heavy-chain variable region primers ( Mlu 1)

VH1

5′-(AG)₁₀ ACG CGT G(T/C)C CA(G/C) TCC CAG GT(G/C) CAG CTG GTG-3′

VH2

5′-(AG)₁₀ ACG CGT GTC (T/C)TG TCC CAG GT(A/G) CAG (C/T)TG (C/A)A9-3′

VH3

5′-(AG)₁₀ ACG CGT GTC CAG TGT GAG GTG CAG CTG-3′

VH4

5’-(AG)₁₀ ACG CGT GTC CTG TCC CAG GTG CAG-3′

VH5

5’-(AG)₉ ACG CGT GTC TGT GCC GAA GTG CAG CTG GTG-3′

3′ heavy-chain variable region primer ( Nhe 1)

5′-(AG)₁₀ GCC CTT GGT GCT AGC TGA GGA GAC GG-3′

5′ κ -variable region primers ( Bgl II)

VK1

5′-AT CAC AGA TCT CTC ACC ATG GAC ATG AGG GTC CCC GCT CAG-3′

VK2

5′-AT CAC AGA TCT CTC ACC ATG AGG CTC CCT GCT CAG-3′

VK3

5′-AT CAC AGA TCT CTC ACC ATG G(G/A)G (A/T)CC CC(T/A) GC(T/G) CAG CT-3′

VK4

5′-AT CAC AGA TCT CTC ACC ATG GTG TTG CAG ACC CAG GTC-3′

3′ κ -variable region primer ( Bsi WI)

5′-(AG)₁₀ TGC AGC CAC CGT ACG TTT GAT TTC CA(G/C) CTT-3′