Two Neutralizing Human Anti-RSV Antibodies: Cloning, Expression, and Characterization

Table 2 Limiting dilution cloning of RF-1 ^a and RF-2 ^b

No. Cells/Well	No. Wells	Anti-F Wells n (%)	Wells with Growth n (%)
RF-1
30	48	48 (100)	48 (100)
10	48	48 (100)	48 (100)
3.3	96	27 (28)	68 (71)
1.1	192	17 (9)	112 (58)
0.38	384	18 (5)	116 (30)
RF-2
30	40	15 (37.5)	40 (100)
10	120	22 (18)	120 (100)
3.3	120	9 (7.5)	102 (85)
1.1	120	1 (0.83)	50 (41.6)
0.33	180	5 (2.8)	30 (16.7)

^aIrradiated EL4-B5 cells were plated out at 5 × 10⁴ cells/well in a flat-bottomed 96-well plate. Approximately 24 hr later, RF-1 cells in exponential growth were plated out on the feeder layer at the described concentrations. After 2–3 weeks, the wells were scored for growth and for presence of anti-F activity.
^bCloning of RF-2 by limiting dilution. Done as described above.

ISSN: 1528-3658