Table 3 Methods of mutation analysis
cDNA Number | Substitution | Sense Primer | Antisense Primer | Rapid Detection Method | ASOH Primers |
|---|---|---|---|---|---|
203 | C→Del | 1361-GCCACCACTACGCCCAGCTA-1380 | 1914-TAAGGGTATCAGTACCCAGC-1895 | ASOH | 1695-CCTTTGA C CCCCCGACCT-1702 1695-CCTTTGACCCCCGACCT1701 |
644 | C→A | 3389-CTGATTCACCGAGCCCTGCA-3408 | 3504-TTGAGTGACCCCTTCCCATT-3485 | −PflMI | — |
661 | C→A | 3389-CTGATTCACCGAGCCCTGCA-3408 | 3504-TTGAGTGACCCCTTCCCATT-3485 | −HphI | — |
721 | G→A | 3052-CATCATCCGGGTACCCATGG-3071 | 3916-GCGCCTATAATCCCAGCT-3899 | −NciI | — |
887 | G→A | 3465-TCAAGACCAATGGAGCGGTG-3484 | 4259-CTAGGTCAGGGCAATGAAG-4240 | —BsmAI | — |
970 | C→T | 4063-TCTGGTCCACTTTCTTGGCC-4082 | 4340-CCCAGTGGGGCAGCAGCATG-4321 | (+NsiI) | — |
1249 | T→G | 5183-CAAGGTCCAGGATCAGTTGC-5202 | 6487-TTTAGCACGACCACAACAGC-6468 | ASOH | 5854-TGTGGTCGGCTGGACCG-5870 5854-TGTGGTCGGCGGGACCG-5870 |
1255 | G→A | 5797-CCAGTGTTGAGCCTTTGTCT-5816 | 5892-GGGTTC AGGGCCAGGTTCC A-5873 | (+XcmI) |