Fig. 6

TNF promoter responses to different LPS concentrations in the presence of dominant inhibitors of ras and raf-1 or in the presence of raf-BXB

Cells were cotransfected with ras, raf-1 (A), or raf-BXB (B) expression constructs or empty vector (control) and with a TNF promoter-CAT construct as described in Materials and Methods. Twenty-four hours later, they were induced with LPS at the concentration indicated. Forty-eight hours post-transfection, the cells were lysed and CAT activity was measured. The relative activity of CAT in stimulated cells, with respect to the activity in control cells, is presented. A separate control was used for each series of cotransfections.