Fig. 2
From: IL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, from prostate tumors
Migration and invasion assays assessing the effects of PEc secreted by the wt and modified PC-3 cells, on MSC mobilization. a and b: Media obtained from PC-3 cells co-incubated with sensitized lymphocytes or with cells of the human innate IR, induced HMSC migration and invasion. This effect was suppressed by the introduction of the anti-PEc antibody (1/100, 30 min) in both cases for both cell lines. c and d: The media obtained from PC-3 IGF-1Ec KD cells after co-incubation with sensitized lymphocytes, presented a significantly lower effect on the migration and invasion capacity of the MSC compared to the media obtained from PC-3 cells under the same treatment. Similar results were observed for DU-145 cells. E: Detection of mouse MSC in human tumors in SCID mice as CD-45 negative cells that posses mouse centromeres, in 1 and 4 weeks. The arrows indicate fusion between mouse and human cells (**: p < 0.005, ***:p < 0.0005)