Uncoupling of glycolysis from glucose oxidation accompanies the development of heart failure with preserved ejection fraction

Table 3 Effect of insulin (100 μU/ml) on cardiac metabolism in Dahl salt-sensitive rats fed a high salt diet (HSD)

	Control	3 weeks	6 weeks	9 weeks
Glycolysis	1231.3 ±234.8	2390.9 ±390.4	2090.6 ±647.2	2982.2 ±734.1
Glucose Oxidation	312.2 ±20.6	519.0 ±119.7	464.8 ±113.6	493.0 ±166.7
Palmitate Oxidation	983.5 ±47.5	931.0 ±38.2	796.9 ±43.8	616.3 ±56.9^*
Lactate Oxidation	377.0 ±46.2	426.5 ±95.0	339.6 ±62.4	275.0 ±113.7
Proton Production	1838.3 ±454.1	3743.8 ±558.6	3251.6 ±1073.8	4978.3 ±1197.6
ATP Production
Glycolysis	2.5 ±0.5	4.8 ±0.8	4.2 ±1.3	6.0 ±1.5
Glucose Oxidation	9.1 ±0.6	15.1 ±3.5	13.5 ±3.3	14.3 ±4.8
Palmitate Oxidation	102.3 ±4.9	96.8 ±4.0	82.9 ±4.6	64.1 ±5.9^*#
Lactate Oxidation	5.5 ±0.7	6.2 ±1.4	4.9 ±0.9	4.0 ±1.6
Total	115.6 ±3.3	122.8 ±6.2	106.4 ±10.2	88.3 ±11.0

Energy metabolic rates (nmol●g dry wt⁻¹●min⁻¹) were measured during the working heart perfusion. Contribution to ATP production (μmol●g dry wt^− 1●min^− 1) was calculated from the metabolic rates assessed via the isolated working heart perfusion in Dahl salt-sensitive rats. These results are from Dahl salt-sensitive rats fed a low salt diet, 0.3% NaCl (Control), or a high salt diet, 8% NaCl, for 3, 6, or 9 weeks. n = 3–5 * p < 0.05 compared to Control. # p < 0.05 compared to 3 weeks. Values shown as mean ±SEM

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