Skip to main content

Table 1 In vitro and in vivo experiments investigating the influence of irradiation on angiogenesis

From: Effects of radiation on the metastatic process

Radiation

  

Vessel origin

Host

Result

 

Molecular

Ref

Target

Mode

Dose

  

Type

IR/Ctrl

  

Endothelial cells

 

C-ion

0.1–8 Gy

HUVEC

Transwell chamber

Migration

< 1

αvβ3; MMP-2

(Takahashi et al., 2003)

    

Collagen

Tube formation

< 1

  
 

kV

0.1–8 Gy

 

Transwell chamber

 

> 1

  
    

Collagen

Tube formation

1

  
 

nm

4 Gy

HUVEC

Matrigel

Tube formation

0.8

αvβ3

(Abdollahi et al., 2005)

 

nm

8 Gy

HMEC

Matrigel

Invasion

1

 

(Kaliski et al., 2005)

 

250 kV

6 Gy

HUVEC

Tissue culture plastic

Wound healing migration

4

eNOS

(Sonveaux et al., 2003)

   

HUVEC

Matrigel

Tube formation

   
 

137 Cs

3 Gy

HUVEC

Matrigel

Tube formation

0.8

αvβ3

(Albert et al., 2006)

 

125 kV

15 Gy

HUVEC

Tissue culture plastic

Wound healing migration

0.6

 

(Imaizumi et al., 2010)

 

6 MV

0.5 Gy

HUVEC

 

Wound healing migration

> 1

VEGFR

(Sofia Vala et al., 2010)

Cancer cells

 

6MV

10 Gy

HUVEC

Transwell/CM C6

Chemotactic migration

2

MMP-2 & -9

(Parthymou et al., 2004)

 

6 MV

10 Gy

CAM

CAM/C6

angiogenesis

> 1

 

(Parthymou et al., 2004)

   

HUVEC

Matrigel/PC3

Invasion

1.9

 

(Abdollahi et al., 2005)

  

8 Gy

HMEC

Matrigel/CM B16

Invasion

2.4

MMP-2

(Kaliski et al., 2005)

 

150 kV

10 Gy

HMEC

Matrigel/CM IOMM

Tube formation

1.4

uPA

(Kargiotis et al., 2008)

 

nm

8 Gy

HMEC

T. c. plastic/CM U251

Branching

1.4

MMP-2

(Badiga et al., 2011)

CAF

 

15 MV

18 Gy

HUVEC

Transwell/CM CAF

Migration

0.6

 

(Hellevik et al., 2013)

   

HUVEC

Matrigel/CM CAF

Tube formation

1

 

(Hellevik et al., 2013)

Inoculation site

 

50 kV

19 Gy

CAM

CAM/C6

Angiogenesis

> 1(48 h)

 

(Polytarchou et al., 2004)

 

6 MV

2 Gy

SCID mouse subcutis

HepG2

Micro-vessel density

> 1

VEGF

 
 

125 kV

20 Gy

C57/Bl subcutis

Matrigel plus FGF2

Micro-vessel density

0.36

FGF2

(Imaizumi et al., 2010)

    

Matrigel plus VEGF

Micro-vessel density

0.11

VEGF

 
 

6 MV

0.5 Gy

nude mouse subcutis

Matrigel plus FGF2

Angiogenesis

> 1

 

(Sofia Vala et al., 2010)

 

125 kV

20 Gy

mammary fat pad.

Mammary/AT1

Micro-vessel density

0.44

 

(Kuonen et al., 2012b)

Total body

 

220 kV

15 Gy

C57/Bl aortic

Tissue culture plastic

Sprouting

0.11

TGFΙβ

(Imaizumi et al., 2010)

 

6 MV

0.5 Gy

zebra fish

Embryo

Angiogenesis

> 1

 

(Sofia Vala et al., 2010)

Tumor

 

250 kV

6 Gy

C57/Bl6 subcutis

LLC

Micro-vessel density

> 1

 

(Sonveaux et al., 2003)

 

6MV

8 Gy

C57/BL6 cerebrum

ALTS1C1

Micro-vessel density

> 1

 

(Wang et al., 2013)

   

SCID mouse subcutis

SW410

Micro-vessel density

2

 

(Timaner et al., 2015)

  1. Abbreviations: avb3, integrin; ALTS1C1, SV40 large T-transformed astrocytes; B16, mouse melanoma cells; C57/BL, mouse strain; C6, rat glioma cells; CAF, cancer-associated fibroblasts; CAM, chick chorioallantoic membrane; C-ion, carbon ion; CM, conditioned medium; eNOS, endothelial nitric oxide synthase; FGF2, fibroblast growth factor 2; HepG2, human hepatoma cells; HMEC, human microvascular endothelial cells; HUVEC, human umbilical vein endothelial cells; IR/Ctrl, irradiated over unirradiated control target; IOMM, human meningioma cells; LLC, Lewis lung cancer cells; MMP, matrix metalloproteinase; MVD, microvessel density; nm, not mentioned; PC3, human prostate cancer cells; pcb, polycarbonate transwell chamber; SW410, human colon carcinoma cells; t.c., tissue culture; TGFb, transforming growth factor beta; U252, human glioma cells; uPA, urokinase-type plasminogen activator; VEGF, vascular endothelial growth factor; VEGFR, vascular endothelial growth factor receptor; WH, wound healing