Fig. 4

Flow cytometry analysis of the distribution of lymphocytes in the spleens of GF + PBS mice, GF + B6 mice and GF + SLE mice. (A) Plots of B cells and B1 cells (top) and percentages of B cells and B1 cells (5 mice per group, bottom). (B) Plots of plasma cells (PCs) and plasma blast cells (PBs; top) and percentages of PCs and PBs (5 mice per group; bottom). (C) Histograms of RORγt⁺ cells to identify the gate strategy. The number inside the histogram is the percentage of RORγt⁺ cells as of live cells (top); Plots of CD4⁺RORγt⁺T cells gated on CD3e⁺CD4⁺ T cells (middle); and percentages of CD4⁺RORrt⁺ T cells (5 mice per group). (D) Plots regulatory T cells (Tregs, left) and percentages of Tregs in 3 groups (5 mice per group; right). Data represent one of three independent experiments. A Mann-Whitney t-test was used to compare groups, and error bars represent means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001