Fig. 4

Myofibroblasts were detected in the peritoneal membrane samples of all groups by the expression of α-smooth muscle actin (α-SMA), using immunohistochemistry (× 200). No α-SMA expression was detected in the peritoneal membrane of the Control (a) or CKD group (b). CG induced PF was associated with a marked increase in α-SMA expression in the PF (c) and CKD/PF groups (d). Animals with PF and uremia treated with TAM (e) or rBMP7 (f) exhibited important reduction in the number of myofibroblasts, as demonstrated via quantitative analysis (g). Overall ANOVA p < 0.0001. Post-test p-values: *p < 0.01 vs Control; ^#p < 0.01 vs CKD, ^†p < 0.01 vs PF, ^§p < 0.01 vs PF/CKD