Fig. 2
From: A novel Mcl-1 inhibitor synergizes with venetoclax to induce apoptosis in cancer cells
MI-238 selectively induces apoptosis in Mcl-1 proficient cells. A Western blotting analysis of indicated protein expressions in cell lysate derived from H1299 parental or Mcl-1 knockout (KO) cells. B H1299 parental or Mcl-1 KO cells were treated with or without 20 μM MI-238 for 48 h, and the cell apoptosis were analyzed by annexin V staining. C Western blot analysis as above were performed in MEF wild-type (WT) or Mcl-1 KO cells. D Apoptosis analysis as above were performed in MEF wild-type (WT) or Mcl-1 KO cells. E, F Cell apoptosis were analyzed by caspase 3 cleavage in H1299 (E) or MEF (F). Data are represented as mean ± SD from three independent replicates, ***P < 0.001 by two-tailed t-test