Fig. 4
From: A novel Mcl-1 inhibitor synergizes with venetoclax to induce apoptosis in cancer cells
MI-238 disrupts the association of Mcl-1 with BH3-only proteins. A Molm13 cells were treated with indicated concentrations with MI-238 for 24 h, followed by Immunoprecipitation (IP) assay using anti-Mcl-1, anti-Bcl2 and anti-Bcl-xL antibodies and western blot analysis with indicated BH3-only proteins. B Molm13 cells were treated with indicated concentrations of MI-238, followed by flow cytometry analysis of Bak activation using the antibody (clone: G317-2) specifically recognized activated Bak. Data are presented as mean ± SD from three independent replicates, ****P < 0.0001 by two-tailed t-test. C Molm13 cells were treated with or without 20 μM MI-238 for 24 h. Then, subcellular fractionation was subsequently performed and the cytochrome C level in mitochondria and cytosol were analyzed by western blot