Fig. 4
Azan trichrome staining for collagen in rat liver sections following serial administrations of CCl4 and after treatment with hADSC disc. A Staining for collagen was absent in the control livers treated with olive oil for 16 weeks. B Serial administrations of CCl4 for 8 weeks produced well developed fibrosis and early cirrhosis with deposition of mature collagen fibers (arrows). C At 16th week of CCl4 administration, the collagen fibers became thick and more prominent with well developed cirrhosis (arrows). D Treatment with hADSC disc for 8 weeks during CCl4 administration resulted in more than 50% decrease of collagen fibers and incomplete fibrous septa (arrows). The hADSC disc which is surgically implanted on right lateral lobe of the liver is visible at the bottom portion. E Marked reduction of cirrhosis on left lateral lobe of the liver in which the animal is treated with hADSC disc on right lateral lobe. The collagen fibers were thin with incomplete fibrous septa and partial bridging (arrows). Original magnification, × 40. F The staining intensity of total collagen was quantified using Image-Pro Discovery software. The data are mean ± S.D. of 10 randomly selected microscopic fields from five rats per group