Fig. 5
Immunohistochemical staining for transforming growth factor-β1 (TGF-β1) in rat liver sections following serial administrations of CCl4 and after treatment with hADSC disc. A Staining for TGF-β1 was completely absent in the control livers treated with olive oil for 16 weeks. B Marked staining for TGF-β1 was present in the fibrotic areas of rat livers treated with CCl4 for 8 weeks (arrows). C Prominent and conspicuous staining for TGF-β1 was present at 16th week of CCl4 administration, especially in the fibrotic zone (arrows). D Treatment with hADSC disc for 8 weeks showed marked reduction in staining for TGF-β1 in the hepatic parenchyma (arrows). The implanted hADSC disc is visible at the bottom portion of the image with positive staining for TGF-β1. E There was significant decrease in staining of TGF-β1 in the left lateral lobe, where the right lateral lobe is implanted with hADSC disc (arrows). A–C Original magnification, × 100. D, E Original magnification, × 40. F The staining intensity of TGF-β1 was quantified using Image-Pro Discovery software. The data are mean ± S.D. of 10 randomly selected microscopic fields from five rats per group