Fig. 7
Immunohistochemical staining for collagen type I in rat liver sections following serial administrations of CCl4 and after treatment with hADSC disc. A Staining for collagen type I was absent in the hepatic parenchyma of control livers treated with olive oil for 16 weeks. B At 8th week of CCl4 administration, there was marked staining for collagen type I in the fibrotic zone indicating deposition of newly formed collagen (arrows). C At 16th week, the staining for collagen type I became more prominent depicting thick collagen fibers (arrows). D Treatment with hADSC disc for 8 weeks resulted in marked decrease of collagen type I staining indicating decreased synthesis and deposition of collagen type I (arrows). The hADSC disc with weak staining for collagen type I is visible at the bottom portion. E Marked reduction of staining for collagen type I in the left lateral lobe, where the right lateral lobe is implanted with hADSC disc. The collagen type I fibers were thin with incomplete fibrous septa and partial bridging (arrows). Original magnification, × 40. F The staining intensity of collagen type I was quantified using Image-Pro Discovery software. The data are mean ± S.D. of 10 randomly selected microscopic fields from five rats per group