Fig. 8
Immunohistochemical staining for collagen type III in rat liver sections following serial administrations of CCl4 and after treatment with hADSC disc. A Staining for collagen type III was completely absent in the control livers treated with olive oil for 16 weeks. B Prominent and marked staining for collagen type III was present in the fibrotic septa of all the rats treated with CCl4 for 8 weeks (arrows). C At 16th week of CCl4 administration, remarkable and conspicuous staining for collagen type III with thick fibers were present (arrows). D Treatment with hADSC disc for 8 weeks depicted marked reduction in staining for collagen type III with feeble and incomplete collagen fibers (arrows). E Significant decrease of staining for collagen type III in the left lateral lobe, where the right lateral lobe is implanted with hADSC disc. Collagen fibers were thin with incomplete fibrous septa and partial bridging (arrows). Original magnification, × 40. F The intensity of collagen type III staining was quantified using Image-Pro Discovery software. The data are mean ± S.D. of 10 randomly selected microscopic fields from five rats per group