Fig. 6

OxLDL upregulated VEGF-A expression and secretion via OLR-1/PI3K/Akt signalling. A, B p-AKT and HIF-1α expression in each group of hEECs treated with oxLDL (50 μg/ml) or PBS for 48 h was examined by Western blots. The histogram represents the greyscale of each lane from the Western blot. The number in each group was 3. * represents p < 0.05. C, D p-AKT and HIF-1α expression in the hEECs treated with oxLDL (50 μg/ml) or PBS for 48 h was examined by immunofluorescence. E, F VEGF-A expression in each group of hEECs treated with oxLDL (50 μg/ml) for 48 h, the AKT inhibitor perifosine (20 mM) and HIF-1α LW6 (20 mM) was examined by Western blotting. The histogram represents the greyscale of each lane from the Western blot. The number in each group was 3. *represents p < 0.05