Fig. 2

TOP2A knockdown inhibits proliferation, causes cell cycle arrest, and induces apoptosis in trophoblasts. A TOP2A expression was analysed after specific sh-RNA transfection. B mRNA levels of TOP2A were detected in JEG-3 cells after the indicated treatment. C The effect of TOP2A knockdown on the protein levels of cleaved-caspase3 and caspase3 were analysed using western blot analysis. D q-PCR was used to analyse the mRNA levels of Ki67, Caspase7, Bax, and Bim after TOP2A knockdown. E The effects of TOP2A on cell proliferation was assessed using EdU assay. F TUNEL assay was used to assess the effect of TOP2A knockdown on apoptosis. G Apoptosis was assessed via flow cytometry analysis. H Changes in cell cycle in treated JEG-3 cells. Each in vitro experiment was repeated three times, and data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns no statistical difference. Scale bar: 100 μm